CRFK
Export
Crandell Reese Feline Kidney, cat kidney cortex epithelial cell
Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
feline
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
V
T-019
1e6
60%
80%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
V-017
1e6
65%
60%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
T-019
3e6
60%
80%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
V-017
3e6
70%
65-70%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
T-020
3e6
70%
80%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
V-013
3e6
80%
80%
Plasmid (general)
2 µg
100 µl
I/II/2b