cells

teratocarcinoma, embryonal carcinoma, epithelial

Cell Type:

Embryonic/Extraembryonic Epith.

Tissue Origin:

Species:

mouse

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Adherent

Recommended Media

MEM Alpha Eagle

MEM Alpha was developed in 1971 and differs from standard MEM by containing the following unique components: vitamin B12, ascorbic acid, non-essential amino acids, sodium pyruvate, lipoic acid, and D-biotin.
This media has been used to culture bone marrow cells and amniotic cells for chromosome analysis.

MEM Alpha is available as:
12-169 - without L-glutamine, deoxyribonucleosides or ribonucleosides
BE02-002 - with Ultraglutamine, deoxyribonucleosides and ribonucleosides

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	CA-136	1e5	89.4-91.4%	90-102%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	CA-136	5e5	89-93%	89-101%	Plasmid (general)	2 µg	100 µl	4D X-Unit
V	C-020	2e6	83-87%	70-90%	Plasmid (general)	2 µg	100 µl	I/II/2b

P19

Recommended Media

Transfection Information