NCI-H1299 [H1299]

lung cancer, epithelial

Cell Type:
Respiratory Tract Epith.
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Respiratory Research
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF EW-127 1e5 98-100% 69-85% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF EW-127 5e5 98% 67-89% Plasmid (general) 2 µg 100 µl 4D X-Unit
C X-005 5e5 99% 70-80% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
McNeill DR, Wilson DM 3rd 
In:
Mol Cancer Res (2007) 5(1): 61 - 70 
Categories:
Transfection 
Authors:
Srsen V, Gnadt N, Dammermann A, Merdes A 
In:
J Cell Biol (2006) 174(5): 625-30