cells

Cells of the visceral epithelium in the kidneys and form a crucial component of the glomerular filtration barrier by covering the exterior basement surface of the glomerular capillary and maintaining a massive filtration surface.

Cell Type:

Kidney Epith.

Tissue Origin:

kidney

Species:

mouse

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	Epithelial, basic	T-020	1e6	70%	80%	Plasmid (general)	3 µg	100 µl	I/II/2b

Citations

Viability of primary cultured podocytes is associated with extracellular high glucose-dependent autophagy downregulation

Categories:

Transfection

Authors:

Audzeyenka I, Rogacka D, Piwkowska A, Angielski S, Jankowski M.

In:

Mol Cell Biochem (2017) 430(1-2): 11-19

Retinoic acid utilizes CREB and USF1 in a transcriptional feed-forward loop in order to stimulate MKP1 expression in human immunodeficiency virus-infected podocytes

Categories:

Transfection

Authors:

Lu TC, Wang Z, Feng X, Chuang P, Fang W, Chen Y, Neves S, Maayan A, Xiong H, Liu Y, Iyengar R, Klotman PE, He JC

In:

Mol Cell Biol (2008) 28(18): 5785-94

HIV-1 Nef disrupts the podocyte actin cytoskeleton by interacting with diaphanous interacting protein (DIP)

Categories:

Transfection

Authors:

Lu TC, He JC, Wang Z, Feng X, Fukumi-Tominaga T, Chen N, Xu J, Iyengar R, Klotman PE

In:

J Biol Chem (2008) 283(13): 8173-82

Podocyte, mouse

Transfection Information

Citations