NK3.3

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NK cell line

Cell Type:
Natural Killer (NK)
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R U-001 5e6 56% 80% Plasmid (general) 5 µg 100 µl I/II/2b

Citations

Three Structurally and Functionally Divergent Kinds of Promoters Regulate Expression of Clonally Distributed Killer Cell Ig-Like Receptors (KIR), of KIR2DL4, and of KIR3DL3
Categories:
Transfection 
Authors:
Trompeter HI, Gomez-Lozano N, Santourlidis S, Eisermann B, Wernet P, Vilches C and Uhrberg M 
In:
J Immunol (2005) 174(7): 4135-4143 
Characterization and analysis of the proximal Janus Kinase 3 promoter
Categories:
Transfection 
Authors:
Aringer M, Hofmann S, Frucht DM, Chen M, Centola M, Morinobu A, Visconti R, Kastner DL, Smolen JS and O'Shea JJ 
In:
J Immunol (2003) 170(12): 6057-6064 
Rapid and highly efficient gene transfer into natural killer cells by nucleofection
Categories:
Transfection 
Authors:
Trompeter HI, Weinhold S, Thiel C, Wernet P and Uhrberg M 
In:
J Immunol Methods (2003) 274: 245-256 
Crucial role of DNA methylation in determination of clonally distributed killer cell Ig-like receptor expression patterns in NK cells
Categories:
Transfection 
Authors:
Santourlidis S, Trompeter HI, Weinhold S, Eisermann, Meyer KL, Wernet P and Uhrberg M 
In:
J Immunol (2002) 169: 4253-4261 

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