A-10

aorta, thoracic, smooth muscle cell

Cell Type:
Smooth muscle cell
Tissue Origin:
aortic
Species:
rat
Research Area:
Cardiovascular
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
L D-030 5e5 61-67% 68-80% Plasmid (general) 2 µg 100 µl I/II/2b
SE CA-135 1e5 91% ±2 52.6% ±9 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE CA-135 5e5 89% ±4 64% ±9 Plasmid (general) 2 µg 100 µl 4D X-Unit