BHK-21

kidney, fibroblast like
Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
hamster
Research Area:
Gastroenterology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
L A-031 1e6 85% ±3 78% ±6 Plasmid (general) 2 µg 100 µl I/II/2b
SE CA-137 1e6 87% ±1 103% ±3 Plasmid (general) 2 µg 100 µl 4D X-Unit
SE CA-137 2e5 86% ±1 75% ±13 Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations

Categories:
Transfection 
Authors:
Fan Z, Li W, Lee SR, Meng Q, Shi B, Bunch TD, White KL, Kong IK, Wang Z. 
In:
PLoS ONE (2014) 9(10): 1-9