BHK-21

kidney, fibroblast like
Cell Type:
Kidney Epith.
Tissue Origin:
kidney
Species:
hamster
Research Area:
Gastroenterology
Cell Characteristics:
Adherent

Recommended Media

Glasgow Minimal Essential Medium (G-MEM) was originally developed to support the growth of baby hamster kidney (BHK-21) cells.  The formula is an Eagle's basal medium modified to contain 2X the normal concentration of amino acids and vitamins.

Storage = 2ºC to 8ºC

PC-1™ is a low-protein, serum-free medium intended for the culture of primary cells and anchorage-dependent cell lines. PC-1 is formulated in a specially modified DMEM/F12 base and contains a complete HEPES buffering system with known amounts of insulin, transferrin, fatty acids, and proprietary proteins assembled under strict quality control procedures. PC-1™ is intended for a variety of research and industrial applications and is formulated using defined components for optimal cell growth, while maintaining the lowest possible protein content.
PC-1™ does not contain L-glutamine.

PC-1™ Liquid Base Medium is to be stored at 2°C-8°C.
The Supplement should be stored at -20°C.
When these two components are combined, the resulting PC-1™ Complete Medium is stable for 45 days at 2°C-8°C.

Once thawed, the appropriate volume of one vial of PC-1™ Supplement must be combined with the companion volume of PC-1™ Liquid Base Medium. Partial reconstitution or repeated freezing and thawing of the PC-1™ Supplement is not advised.

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
L A-031 1e6 85% ±3 78% ±6 Plasmid (general) 2 µg 100 µl I/II/2b
SE CA-137 1e6 87% ±1 103% ±3 Plasmid (general) 2 µg 100 µl 4D X-Unit
SE CA-137 2e5 86% ±1 75% ±13 Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations

Categories:
Transfection 
Authors:
Fan Z, Li W, Lee SR, Meng Q, Shi B, Bunch TD, White KL, Kong IK, Wang Z. 
In:
PLoS ONE (2014) 9(10): 1-9