cells

kidney, fibroblast like

Cell Type:

Kidney Epith.

Tissue Origin:

kidney

Species:

hamster

Research Area:

Gastroenterology

Cell Characteristics:

Adherent

Recommended Media

Glasgow Minimal Essential Medium

Glasgow Minimal Essential Medium (G-MEM) was originally developed to support the growth of baby hamster kidney (BHK-21) cells. The formula is an Eagle's basal medium modified to contain 2X the normal concentration of amino acids and vitamins.

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
L	A-031	1e6	85% ±3	78% ±6	Plasmid (general)	2 µg	100 µl	I/II/2b
SE	CA-137	1e6	87% ±1	103% ±3	Plasmid (general)	2 µg	100 µl	4D X-Unit
SE	CA-137	2e5	86% ±1	75% ±13	Plasmid (general)	0.4 µg	20 µl	4D X-Unit

Citations

Efficient gene targeting in golden Syrian hamsters by the CRISPR/Cas9 system

Categories:

Transfection

Authors:

Fan Z, Li W, Lee SR, Meng Q, Shi B, Bunch TD, White KL, Kong IK, Wang Z.

In:

PLoS ONE (2014) 9(10): 1-9