DU 145

prostate carcinoma cell line derived from metastatic site of brain, epithelial like

Cell Type:
Prostate Epith.
Tissue Origin:
brain
Species:
human
Research Area:
Cancer Research/Cell Biology
Neurobiology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE CA-137 1e6 86-94% 63-121% Plasmid (general) 2 µg 100 µl 4D X-Unit
SE CA-137 2e5 84-94% 85-87% Plasmid (general) 400 ng 20 µl 4D X-Unit
L A-023 1e6 44-50% 87-91% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Chen CS, Wang YC, Yang HC, Huang PH, Kulp SK, Yang CC, Lu YS, Matsuyama S, Chen CY, Chen CS 
In:
Cancer Res (2007) 67(11): 5318-27 
Categories:
Transfection 
Authors:
Park C, Lee I, Kang WK 
In:
Mol Cancer Ther (2006) 5(12): 3191-6 
Categories:
Live Cell Imaging 
Authors:
Orellana-Serradell O1, Herrera D1, Castellon EA1, Contreras HR1. 
In:
Asian J Androl () 3: 294-299