EL4

T-lymphocyte, lymphoma

Cell Type:
T Cell
Tissue Origin:
blood
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

HL-1™ is a Serum-free culture medium containing less than 30 µg protein per ml. Components of HL-1 include known amounts of insulin, a variety of saturated and unsaturated fatty acids and proprietary stabilizing bovine proteins. It contains no bovine serum albumin and does not contain L-glutamine.
HL-1™ will support the serum-free growth of various hybridomas and certain other differentiated cells of lymphoid origin. 

Storage = 2°C to 8°C

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF CM-120 1e6 66-92% 41-73% Plasmid (general) 2 µg 100 µl 4D X-Unit
SF CM-120 2e5 73-91% 70-88% Plasmid (general) 400 ng 20 µl 4D X-Unit
L C-009 2e6 61-69% 70-82% Plasmid (general) 2 µg 100 µl I/II/2b
SE DS-113 4e5 70-80% good Plasmid (general) 400 ng 20 µl Shuttle
SE DS-120 4e5 70-80% good Plasmid (general) 400 ng 20 µl Shuttle

Citations (10)

Categories:
Transfection 
Authors:
Lei J, Osen W, Gardyan A, Hotz-Wagenblatt A, Wei G, Gissmann L, Eichmüller S, Löchelt M. Guochao Wei, Lutz Gissmann, Stefan Eichmüller, Martin Löchelt 
In:
PLoS ONE (2015) 10(9): e0138458 
Categories:
Transfection 
Authors:
Takaki H, Ichiyama K, Koga K, Chinen T, Takaesu G, Sugiyama Y, Kato S, Yoshimura A, Kobayashi T 
In:
J Biol Chem (2008) 283(22): 14955-62 
Categories:
Transfection 
Authors:
Lewis MD, Miller SA, Miazgowicz MM, Beima KM, Weinmann AS 
In:
Mol Cell Biol (2007) 27(24): 8510-21 
Categories:
Transfection 
Authors:
Jen KY, Campo M, He H, Makani SS, Velasco G, Rothstein DM, Perkins DL, Finn PW 
In:
J Immunol (2007) 179(6): 4212-8 
Categories:
Transfection 
Authors:
Gocke AR, Cravens PD, Ben LH, Hussain RZ, Northrop SC, Racke MK, Lovett-Racke AE 
In:
J Immunol (2007) 178(3): 1341-8 
Categories:
Transfection 
Authors:
Eguchi J, Hatano M, Nishimura F, Zhu X, Dusak JE, Sato H, Pollack IF, Storkus WJ, Okada H 
In:
Cancer Res (2006) 66(11): 5883-91 
Categories:
Transfection 
Authors:
Zhang Y, Baig E, Williams DB 
In:
J Biol Chem (2006) 281(21): 14622-31 
Categories:
Transfection 
Authors:
Beima KM, Miazgowicz MM, Lewis MD, Yan PS, Huang TH, Weinmann AS 
In:
J Biol Chem (2006) 281(17): 11992-2000 
Categories:
Transfection 
Authors:
Choi EY, Newman AE, Burger L and Pintel D 
In:
J Virol (2005) 79(19): 12375-12381 
Categories:
Transfection 
Authors:
Liu WH and Lai MZ 
In:
Mol Cell Biol (2005) 25(4): 1367-1378 
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