EL4

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T-lymphocyte, lymphoma

Cell Type:
T Cell
Tissue Origin:
blood
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF CM-120 1e6 66-92% 41-73% Plasmid (general) 2 µg 100 µl 4D X-Unit
SF CM-120 2e5 73-91% 70-88% Plasmid (general) 400 ng 20 µl 4D X-Unit
L C-009 2e6 61-69% 70-82% Plasmid (general) 2 µg 100 µl I/II/2b
SE DS-113 4e5 70-80% good Plasmid (general) 400 ng 20 µl 96-well
SE DS-120 4e5 70-80% good Plasmid (general) 400 ng 20 µl 96-well

Citations (10)

Replication-Competent Foamy Virus Vaccine Vectors as Novel Epitope Scaffolds for Immunotherapy
Categories:
Transfection 
Authors:
Lei J, Osen W, Gardyan A, Hotz-Wagenblatt A, Wei G, Gissmann L, Eichmüller S, Löchelt M. Guochao Wei, Lutz Gissmann, Stefan Eichmüller, Martin Löchelt 
In:
PLoS ONE (2015) 10(9): e0138458 
STAT6 inhibits TGF-beta 1-mediated Foxp3 induction through direct binding to the Foxp3 promoter, which is reverted by retinoic acid receptor
Categories:
Transfection 
Authors:
Takaki H, Ichiyama K, Koga K, Chinen T, Takaesu G, Sugiyama Y, Kato S, Yoshimura A, Kobayashi T 
In:
J Biol Chem (2008) 283(22): 14955-62 
T-bet's ability to regulate individual target genes requires the conserved T-box domain to recruit histone methyltransferase activity and a separate family member specific transactivation domain
Categories:
Transfection 
Authors:
Lewis MD, Miller SA, Miazgowicz MM, Beima KM, Weinmann AS 
In:
Mol Cell Biol (2007) 27(24): 8510-21 
CD45RB Ligation Inhibits Allergic Pulmonary Inflammation by Inducing CTLA4 Transcription
Categories:
Transfection 
Authors:
Jen KY, Campo M, He H, Makani SS, Velasco G, Rothstein DM, Perkins DL, Finn PW 
In:
J Immunol (2007) 179(6): 4212-8 
T-bet regulates the fate of Th1 and Th17 lymphocytes in autoimmunity
Categories:
Transfection 
Authors:
Gocke AR, Cravens PD, Ben LH, Hussain RZ, Northrop SC, Racke MK, Lovett-Racke AE 
In:
J Immunol (2007) 178(3): 1341-8 
Identification of Interleukin-13 Receptor 2 Peptide Analogues Capable of Inducing Improved Antiglioma CTL Responses
Categories:
Transfection 
Authors:
Eguchi J, Hatano M, Nishimura F, Zhu X, Dusak JE, Sato H, Pollack IF, Storkus WJ, Okada H 
In:
Cancer Res (2006) 66(11): 5883-91 
Functions of ERp57 in the folding and assembly of MHC Class I molecules
Categories:
Transfection 
Authors:
Zhang Y, Baig E, Williams DB 
In:
J Biol Chem (2006) 281(21): 14622-31 
T-bet binding to newly identified target gene promoters is cell-type independent, but results in variable context-dependent functional effects
Categories:
Transfection 
Authors:
Beima KM, Miazgowicz MM, Lewis MD, Yan PS, Huang TH, Weinmann AS 
In:
J Biol Chem (2006) 281(17): 11992-2000 
Replication of Minute Virus of Mice DNA Is Critically Dependent on Accumulated Levels of NS2
Categories:
Transfection 
Authors:
Choi EY, Newman AE, Burger L and Pintel D 
In:
J Virol (2005) 79(19): 12375-12381 
Deltex regulates T-cell activation by targeted degradation of active MEKK1
Categories:
Transfection 
Authors:
Liu WH and Lai MZ 
In:
Mol Cell Biol (2005) 25(4): 1367-1378 
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