myeloma, lymphoma morphology

Cell Type:
B cell
Tissue Origin:
Cell Characteristics:

Recommended Media

ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) is universally suitable for cryopreserving many cell types in the absence of fetal bovine serum (FBS), which is particularly advantageous for freezing of cells cultured in a serum-free and animal component-free environment.
This protein-free freezing medium contains no animal derived components, insulin, or hydrolysate, and maintains high cell viability upon recovery from frozen storage.

ProFreeze™ Medium requires the addition of 15% reagent or spectrophotometric grade dimethylsulfoxide (DMSO) at time of use.

Storage = 2°C to 8°C

ProNS0™ 1 CD Medium, together with ProNS0™ Lipid CD Supplement, is designed to meet a growing demand for optimized NS0 formulations.
- Optimized formulation to cover the broadest range of NS0 nutritional needs
- Further maximize protein production by titration with ProNS0™ Lipid CD Supplement
- Protein-free and chemically defined formulations
- Non-animal origin components reduce regulatory burden

Basal media = 2°C to 8°C
Lipid supplement = -10°C to -20°C

12-773 - ProNS0 1
12-775 - ProNS0 lipid chemically defined supplement 

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF DD-100 4e5 81-87% 42-54% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF DD-100 2e6 90-92% 25-39% Plasmid (general) 2 µg 100 µl 4D X-Unit
C T-005 2e6 81-85% 48.8-58.8% Plasmid (general) 2 µg 100 µl I/II/2b