NB-4
Export
Acute promyelocytic leukemia established from the bone marrow of a 23-year-old woman.
Cell Type:
Myeloid (unspec.)
Tissue Origin:
bone marrow
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
V
X-001
2e6
63-79%
59-73%
Plasmid (general)
2 µg
100 µl
I/II/2b
SF
CZ-100
4e5
72%
100%
mRNA
0.8 µg
20 µl
4D X-Unit
SF
CZ-100
4e5
74.4-92.4%
72.5%
mRNA
0.8 µg
20 µl
4D X-Unit
SF
CZ-100
4e5
86.3-90.3%
92.8%
0.4 µg
20 µl
4D X-Unit
SF
CZ-100
4e5
68-76%
73.2%
0.4 µg
20 µl
4D X-Unit
SF
CZ-100
2e6
90.7-94.7%
95.4%
2 µg
100 µl
4D X-Unit
SF
CZ-100
2e6
87.2-93.2%
53.8%
2 µg
100 µl
4D X-Unit
Citations
RNAi screen identifies UBE2D3 as a mediator of all-trans retinoic acid-induced cell growth arrest in human acute promyelocytic NB4 cells
Categories:
Transfection
Authors:
Hattori H, Zhang X, Jia Y, Subramanian KK, Jo H, Loison F, Newburger PE, Luo HR
In:
Blood (2007) 110(2): 640-50
MicroRNA gene expression during retinoic acid-induced differentiation of human acute promyelocytic leukemia
Categories:
Transfection
Authors:
Garzon R, Pichiorri F, Palumbo T, Visentini M, Aqeilan R, Cimmino A, Wang H, Sun H, Volinia S, Alder H, Calin GA, Liu CG, Andreeff M, Croce CM
In:
Oncogene (2007) 26(28): 4148-57
Pharmacogenomic analysis of acute promyelocytic leukemia cells highlights CYP26 cytochrome metabolism in differential all-trans retinoic acid sensitivity
Categories:
Transfection
Authors:
Quere R, Baudet A, Cassinat B, Bertrand G, Marti J, Manchon L, Piquemal D, Chomienne C, Commes T
In:
Blood (2007) 109(10): 4450-60
Programmed cell death-4 tumor suppressor protein contributes to retinoic Acid-induced terminal granulocytic differentiation of human myeloid leukemia cells
Categories:
Transfection
Authors:
Ozpolat B, Akar U, Steiner M, Zorrilla-Calancha I, Tirado-Gomez M, Colburn N, Danilenko M, Kornblau S, Lopez Berestein G
In:
Mol Cancer Res (2007) 5(1): 95-108
Cleavage of misfolded nuclear receptor corepressor confers resistance to unfolded protein response-induced apoptosis
Categories:
Transfection
Authors:
Ng AP, Howe Fong J, Sijin Nin D, Hirpara JL, Asou N, Chen CS, Pervaiz S, Khan M
In:
Cancer Res (2006) 66(20): 9903-12