T-47D

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Mammary gland ductal carcinoma. Isolated by I. Keydar from a pleural effusion obtained from a 54 year old female patient with an infiltrating ductal carcinoma of the breast.

Cell Type:
Mammary Epith.
Tissue Origin:
breast
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE EN-104 2e5 72-74% 64-74% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE EN-104 1e6 81-87% 56-76% Plasmid (general) 2 µg 100 µl 4D X-Unit
V X-005 1e6 48-54% 93-95% Plasmid (general) 2 µg 100 µl I/II/2b
SE 96-FF-150 2e5 80% Plasmid (general) 20 µl 96-well

Citations

Feedback regulation between ZBP1 and beta-catenin mRNAs in breast cancer cells
Categories:
Transfection 
Authors:
Gu W, Wells AL, Pan F, Singer RH 
In:
Mol Cell Biol (2008) 28(16): 4963-74 
STAT5b, c-Src, and EGFR Signaling Play Integral Roles in Estrogen-Stimulated Proliferation of ER-Positive Breast Cancer Cells
Categories:
Transfection 
Authors:
Fox EM, Bernaciak TM, Wen J, Weaver AM, Shupnik MA, Silva CM 
In:
Mol Endocrinol (2008) 22(8): 1781-96 
Selective inhibition of histone deacetylase 2 silences progesterone receptor-mediated signaling
Categories:
Transfection 
Authors:
Bicaku E, Marchion DC, Schmitt ML, Münster PN 
In:
Cancer Res (2008) 68(5): 1513-9 
Breast cancer antiestrogen resistance-3 expression regulates breast cancer cell migration through promotion of p130Cas membrane localization and membrane ruffling
Categories:
Transfection 
Authors:
Schrecengost RS, Riggins RB, Thomas KS, Guerrero MS, Bouton AH 
In:
Cancer Res (2007) 67(13): 6174-82 
R5020 and RU486 Act as Progesterone Receptor Agonists to Enhance Sp1/Sp4-Dependent Gene Transcription by an Indirect Mechanism
Categories:
Transfection 
Authors:
Shatnawi A, Tran T, Ratnam M 
In:
Mol Endocrinol (2007) 21(3): 635-50 
Identification of RAI3 as a therapeutic target for breast cancer
Categories:
Transfection 
Authors:
Nagahata T, Sato T, Tomura A, Onda M, Nishikawa K and Emi M 
In:
Endocr Relat Cancer (2005) 12(1): 65-73 

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