Saos-2

osteosarcoma with epithelial morphology

Cell Type:
Bone
Tissue Origin:
bone
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF EN-137 2e5 76-84% 80-84% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF EN-137 1e6 94% 68-74% Plasmid (general) 2 µg 100 µl 4D X-Unit
V D-024 1e6 78-86% 74-84% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
M Kuhlwilm, A Davierwala, S Pääbo 
In:
PLoS ONE (2013) 8 (12): e83218