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acute myelogenous leukemia (AML) cell line

Cell Type:
Myeloid (unspec.)
Tissue Origin:
bone marrow
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Recommended Media

Iscove's Modified Dulbecco's Medium

Iscove's Modified Dulbecco's Medium (IMDM) is a modified DME containing high glucose (4,500 mg/L), sodium pyruvate, additional amino acids, HEPES buffer (in liquid form), selenium and other components. IMDM is suitable for fast growing cells and can support the growth of a wide variety of mammalian cells.

Storage = 2ºC to 8ºC

12-722 with L-glutamine
12-726 without L-glutamine
12-915 with L-glutamine and screened to support hybridoma growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF FF-100 1e6 63-69% 45-53% Plasmid (general) 5 µg 100 µl 4D X-Unit
SF FF-100 2e5 58-62% 47-55% Plasmid (general) 1 µg 20 µl 4D X-Unit
R V-001 2e6 68-72% 77-91% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Myeloblastic cell lines mimic some but not all aspects of human cytomegalovirus experimental latency defined in primary CD34+ cell populations.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Albright ER1, Kalejta RF 
In:
J Virol (2013) 87(17): 9802-12 
Regulation of cell shape and adhesion by CD34.
Categories:
Primary Cells and Media 
Authors:
Ohnishi H, Sasaki H, Nakamura Y, Kato S, Ando K, Narimatsu H, Tachibana K 
In:
Cell Adh Migr (2013) 7(5): 426-33 
Virally induced changes in cellular microRNAs maintain latency of human cytomegalovirus in CD34? progenitors.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Poole E, McGregor Dallas SR, Colston J, Joseph RS, Sinclair J 
In:
J Gen Virol (2011) 92(7): 1539-49 
Lipid raft adhesion receptors and Syk regulate selectin-dependent rolling under flow conditions
Categories:
Transfection 
Authors:
Abbal C, Lambelet M, Bertaggia D, Gerbex C, Martinez M, Arcaro A, Schapira M, Spertini O 
In:
Blood (2006) 108(10): 3352-9 

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