HT-1080
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Connective tissue fibrosarcoma from acetabulum.
Cell Type:
Fibroblast
Tissue Origin:
bone
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SF
FF-113
1e6
98%
71%
Plasmid (general)
2 µg
100 µl
4D X-Unit
SF
FF-113
2e5
98%
81%
Plasmid (general)
400 ng
20 µl
4D X-Unit
T
L-005
1e6
60-88%
67-85%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Molecular Dissection of the Structural Machinery Underlying the Tissue-invasive Activity of Membrane Type-1 Matrix Metalloproteinase
Categories:
Transfection
Authors:
Li XY, Ota I, Yana I, Sabeh F, Weiss SJ
In:
Mol Biol Cell (2008) 19(8): 3221-33
The glycosyltransferase activities of lysyl hydroxylase 3 (LH3) in the extracellular space are important for cell growth and viability
Categories:
Transfection
Authors:
Wang C, Kovanen V, Raudasoja P, Eskelinen S, Pospiech H, Myllylä R
In:
J Cell Mol Med (2008) epub: online
Bax inhibitor-1 regulates endoplasmic reticulum stress-associated reactive oxygen species and heme oxygenase-I expression
Categories:
Transfection
Authors:
Lee GH, Kim HK, Chae SW, Kim DS, Ha KC, Mike C, Kress C, Reed JC, Kim HR, Chae HJ
In:
J Biol Chem (2007) 282(30): 21618-28
Tumor cell traffic through the extracellular matrix is controlled by the membrane-anchored collagenase MT1-MMP
Categories:
Transfection
Authors:
Sabeh F, Ota I, Holmbeck K, Birkedal-Hansen H, Soloway P, Balbin M, Lopez-Otin C, Shapiro S, Inada M, Krane S, Allen E, Chung D and Weiss SJ
In:
J Cell Biol (2004) 167(4): 769-781