Macrophage, mouse - BALB/c
Export
Bone-marrow derived primary immune cells.
Cell Type:
Macrophage
Tissue Origin:
bone marrow
Species:
mouse
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
Macrophage, mouse
Y-001
1e6
42-48%
88-96%
Plasmid (general)
2 µg
100 µl
I/II/2b
Macrophage, mouse
Y-001
1e6
28-40%
79-89%
Plasmid (general)
2 µg
100 µl
I/II/2b
P2
DO-100
4e5
33-37%
41%
Plasmid (general)
1 µg
20 µl
4D X-Unit
Citations (7)
TRAM couples endocytosis of Toll-like receptor 4 to the induction of interferon-beta
Categories:
Transfection
Authors:
Kagan JC, Su T, Horng T, Chow A, Akira S, Medzhitov R
In:
Nat Immunol (2008) 9(4): 361-8
Phospholipase C beta3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice
Categories:
Transfection
Authors:
Wang Z, Liu B, Wang P, Dong X, Fernandez-Hernando C, Li Z, Hla T, Li Z, Claffey K, Smith JD, Wu D
In:
J Clin Invest (2008) 118(1): 195-204
Ferroxidase activity is required for the stability of cell surface ferroportin in cells expressing GPI-ceruloplasmin
Categories:
Transfection
Authors:
De Domenico I, Ward DM, di Patti MC, Jeong SY, David S, Musci G, Kaplan J
In:
EMBO J (2007) 26(12): 2823-31
Glucocorticoid conditioning of myeloid progenitors enhances TLR4 signaling via negative regulation of the phosphatidylinositol 3-kinase-Akt pathway
Categories:
Transfection
Authors:
Zhang TY, Daynes RA
In:
J Immunol (2007) 178(4): 2517-26
TLR-Dependent Induction of IFN-beta Mediates Host Defense against Trypanosoma cruzi
Categories:
Transfection
Authors:
Koga R, Hamano S, Kuwata H, Atarashi K, Ogawa M, Hisaeda H, Yamamoto M, Akira S, Himeno K, Matsumoto M, Takeda K
In:
J Immunol (2006) 177(10): 7059-66
Reduction in ABCG1 in type 2 diabetic mice increases macrophage foam cell formation
Categories:
Transfection
Authors:
Mauldin JP, Srinivasan S, Mulya A, Gebre A, Parks JS, Daugherty A, Hedrick CC
In:
J Biol Chem (2006) 281(30): 21216-24
Phosphoinositide-mediated adaptor recruitment controls Toll-like receptor signaling
Categories:
Transfection
Authors:
Kagan JC, Medzhitov R
In:
Cell (2006) 125(5): 943-55
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