Macrophage, mouse - C57BL/6

Bone-marrow derived primary immune cells.
Cell Type:
Macrophage
Tissue Origin:
bone marrow
Species:
mouse
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Macrophage, mouse Y-001 1e6 41-53% 85-91% Plasmid (general) 2 µg 100 µl I/II/2b
Macrophage, mouse Y-001 1e6 24% ±6 80% ±6 Plasmid (general) 2 µg 100 µl I/II/2b

Citations (9)

Categories:
Transfection 
Authors:
Gade P, Roy SK, Li H, Nallar SC, Kalvakolanu DV 
In:
Mol Cell Biol (2008) 28(8): 2528-48 
Categories:
Transfection 
Authors:
Kagan JC, Su T, Horng T, Chow A, Akira S, Medzhitov R 
In:
Nat Immunol (2008) 9(4): 361-8 
Categories:
Transfection 
Authors:
Wang Z, Liu B, Wang P, Dong X, Fernandez-Hernando C, Li Z, Hla T, Li Z, Claffey K, Smith JD, Wu D 
In:
J Clin Invest (2008) 118(1): 195-204 
Categories:
Transfection 
Authors:
De Domenico I, Ward DM, di Patti MC, Jeong SY, David S, Musci G, Kaplan J 
In:
EMBO J (2007) 26(12): 2823-31 
Categories:
Transfection 
Authors:
Koga R, Hamano S, Kuwata H, Atarashi K, Ogawa M, Hisaeda H, Yamamoto M, Akira S, Himeno K, Matsumoto M, Takeda K 
In:
J Immunol (2006) 177(10): 7059-66 
Categories:
Transfection 
Authors:
Mauldin JP, Srinivasan S, Mulya A, Gebre A, Parks JS, Daugherty A, Hedrick CC 
In:
J Biol Chem (2006) 281(30): 21216-24 
Categories:
Transfection 
Authors:
Kagan JC, Medzhitov R 
In:
Cell (2006) 125(5): 943-55 
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