Macrophage, mouse - C57BL/6

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Bone-marrow derived primary immune cells.
Cell Type:
Macrophage
Tissue Origin:
bone marrow
Species:
mouse
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Macrophage, mouse Y-001 1e6 41-53% 85-91% Plasmid (general) 2 µg 100 µl I/II/2b
Macrophage, mouse Y-001 1e6 24% ±6 80% ±6 Plasmid (general) 2 µg 100 µl I/II/2b

Citations (10)

Efficient gene knockout in primary human and murine myeloid cells by non-viral delivery of CRISPR-Cas9.
Categories:
Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Freund EC, Lock JY, Oh J, Maculins T, Delamarre L, Bohlen CJ, Haley B, Murthy A. 
In:
J Exp Med (2020) 217(7): e20191692 
Critical role for transcription factor C/EBP-beta in regulating the expression of death-associated protein kinase 1
Categories:
Transfection 
Authors:
Gade P, Roy SK, Li H, Nallar SC, Kalvakolanu DV 
In:
Mol Cell Biol (2008) 28(8): 2528-48 
TRAM couples endocytosis of Toll-like receptor 4 to the induction of interferon-beta
Categories:
Transfection 
Authors:
Kagan JC, Su T, Horng T, Chow A, Akira S, Medzhitov R 
In:
Nat Immunol (2008) 9(4): 361-8 
Phospholipase C beta3 deficiency leads to macrophage hypersensitivity to apoptotic induction and reduction of atherosclerosis in mice
Categories:
Transfection 
Authors:
Wang Z, Liu B, Wang P, Dong X, Fernandez-Hernando C, Li Z, Hla T, Li Z, Claffey K, Smith JD, Wu D 
In:
J Clin Invest (2008) 118(1): 195-204 
Macrophages from 11beta-Hydroxysteroid Dehydrogenase Type 1-Deficient Mice Exhibit an Increased Sensitivity to Lipopolysaccharide Stimulation Due to TGF-beta-Mediated Up-Regulation of SHIP1 Expression
Categories:
Transfection 
Authors:
Zhang TY, Daynes RA 
In:
J Immunol (2007) 179(9): 6325-35 
Ferroxidase activity is required for the stability of cell surface ferroportin in cells expressing GPI-ceruloplasmin
Categories:
Transfection 
Authors:
De Domenico I, Ward DM, di Patti MC, Jeong SY, David S, Musci G, Kaplan J 
In:
EMBO J (2007) 26(12): 2823-31 
Glucocorticoid conditioning of myeloid progenitors enhances TLR4 signaling via negative regulation of the phosphatidylinositol 3-kinase-Akt pathway
Categories:
Transfection 
Authors:
Zhang TY, Daynes RA 
In:
J Immunol (2007) 178(4): 2517-26 
TLR-Dependent Induction of IFN-beta Mediates Host Defense against Trypanosoma cruzi
Categories:
Transfection 
Authors:
Koga R, Hamano S, Kuwata H, Atarashi K, Ogawa M, Hisaeda H, Yamamoto M, Akira S, Himeno K, Matsumoto M, Takeda K 
In:
J Immunol (2006) 177(10): 7059-66 
Reduction in ABCG1 in type 2 diabetic mice increases macrophage foam cell formation
Categories:
Transfection 
Authors:
Mauldin JP, Srinivasan S, Mulya A, Gebre A, Parks JS, Daugherty A, Hedrick CC 
In:
J Biol Chem (2006) 281(30): 21216-24 
Phosphoinositide-mediated adaptor recruitment controls Toll-like receptor signaling
Categories:
Transfection 
Authors:
Kagan JC, Medzhitov R 
In:
Cell (2006) 125(5): 943-55 
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