cells

cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte; from the AT-1 mouse atrial cardiomyocyte tumor lineage

Cell Type:

Cardiac Muscle

Tissue Origin:

heart

Species:

mouse

Research Area:

Cardiovascular

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
V	A-033	3e6	43%	70%	Plasmid (general)	2 µg	100 µl	I/II/2b
V	B-033	3e6	37%		Plasmid (general)	2 µg	100 µl	I/II/2b
V	T-020	2e6	70%	50%	Plasmid (general)	2 µg	100 µl	I/II/2b
SF	DN-100	2e5	70-80%	good	Plasmid (general)	400 ng	20 µl	4D X-Unit
SF	EH-100	2e5	70-80%	good	Plasmid (general)	400 ng	20 µl	4D X-Unit

Citations

Identification and Characterization of a TranscribedDistal Enhancer Involved in Cardiac Kcnh2Regulation

Categories:

Serum-free and Speciality Media

Authors:

Malou van den Boogaard,Jan Hendrik van Weerd,Amira C. Bawazeer, ..., Phil Barnett,Jeroen Bakkers, Vincent M. Christoffels

In:

Cell Rep (2019) 28: 2704-2714

HL-1

Transfection Information

Citations