HMEC-1

SV40-transformed human microvascular endothelial cell line. Derived by Ades et al. [J Invest Dermatol. (1992) 99(6):683-90].

Cell Type:
Microvascular Endo.
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Cardiovascular
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R T-016 1e6 74% 83% Plasmid (general) 5 µg 100 µl I/II/2b
R T-020 1e6 69% 78% Plasmid (general) 5 µg 100 µl I/II/2b

Citations (7)

Categories:
Transfection 
Authors:
Hellebrekers DM, Melotte V, Vire E, Langenkamp E, Molema G, Fuks F, Herman JG, Van Criekinge W, Griffioen AW, van Engeland M 
In:
Cancer Res (2007) 67(9): 4138-48 
Categories:
Transfection 
Authors:
Prabhat P, Gan Z, Chao J, Ram S, Vaccaro C, Gibbons S, Ober RJ, Ward ES 
In:
Proc Natl Acad Sci USA (2007) 104(14): 5889-94 
Categories:
Transfection 
Authors:
Pourroy B, Honore S, Pasquier E, Bourgarel-Rey V, Kruczynski A, Briand C, Braguer D 
In:
Cancer Res (2006) 66(6): 3256-63 
Categories:
Transfection 
Authors:
Vaccaro C, Zhou J, Ober RJ and Ward ES 
In:
Nat Biotechnol (2005) 23(10): 1283-1288 
Categories:
Transfection 
Authors:
Ward ES, Martinez C, Vaccaro C, Zhou J, Tang Q and Ober RJ 
In:
Mol Biol Cell (2005) 16(4): 2028-2038 
Categories:
Transfection 
Authors:
Ober RJ, Martinez C, Lai X, Zhou J and Ward ES 
In:
Proc Natl Acad Sci USA (2004) 101(30): 11076-11081 
Categories:
Transfection 
Authors:
Ober RJ, Martinez C, Vaccaro C, Zhou J and Ward ES 
In:
J Immunol (2004) 172(4): 2021-2029 
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