HMEC-1

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SV40-transformed human microvascular endothelial cell line. Derived by Ades et al. [J Invest Dermatol. (1992) 99(6):683-90].

Cell Type:
Microvascular Endo.
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Cardiovascular
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R T-016 1e6 74% 83% Plasmid (general) 5 µg 100 µl I/II/2b
R T-020 1e6 69% 78% Plasmid (general) 5 µg 100 µl I/II/2b
SF CP-101 2e5 60% ±2 61% ±3 Plasmid (general) 1 µg 20 µl 4D X-Unit
SF CP-101 1e6 72% ±2 48% ±4 Plasmid (general) 5 µg 100 µl 4D X-Unit

Citations (7)

Identification of epigenetically silenced genes in tumor endothelial cells
Categories:
Transfection 
Authors:
Hellebrekers DM, Melotte V, Vire E, Langenkamp E, Molema G, Fuks F, Herman JG, Van Criekinge W, Griffioen AW, van Engeland M 
In:
Cancer Res (2007) 67(9): 4138-48 
Elucidation of intracellular recycling pathways leading to exocytosis of the Fc receptor, FcRn, by using multifocal plane microscopy
Categories:
Transfection 
Authors:
Prabhat P, Gan Z, Chao J, Ram S, Vaccaro C, Gibbons S, Ober RJ, Ward ES 
In:
Proc Natl Acad Sci USA (2007) 104(14): 5889-94 
Antiangiogenic concentrations of vinflunine increase the interphase microtubule dynamics and decrease the motility of endothelial cells
Categories:
Transfection 
Authors:
Pourroy B, Honore S, Pasquier E, Bourgarel-Rey V, Kruczynski A, Briand C, Braguer D 
In:
Cancer Res (2006) 66(6): 3256-63 
Engineering the Fc region of immunoglobulin G to modulate in vivo antibody levels
Categories:
Transfection 
Authors:
Vaccaro C, Zhou J, Ober RJ and Ward ES 
In:
Nat Biotechnol (2005) 23(10): 1283-1288 
From Sorting Endosomes to Exocytosis: Association of Rab4 and Rab11 GTPases with the Fc Receptor, FcRn, during Recycling
Categories:
Transfection 
Authors:
Ward ES, Martinez C, Vaccaro C, Zhou J, Tang Q and Ober RJ 
In:
Mol Biol Cell (2005) 16(4): 2028-2038 
Exocytosis of IgG as mediated by the receptor, FcRn: an analysis at the single-molecule level
Categories:
Transfection 
Authors:
Ober RJ, Martinez C, Lai X, Zhou J and Ward ES 
In:
Proc Natl Acad Sci USA (2004) 101(30): 11076-11081 
Visualizing the site and dynamics of IgG salvage by the MHC class I-related receptor, FcRn
Categories:
Transfection 
Authors:
Ober RJ, Martinez C, Vaccaro C, Zhou J and Ward ES 
In:
J Immunol (2004) 172(4): 2021-2029 
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