cells

gastric adenocarcinoma

Cell Type:

GI Tract Epith.

Tissue Origin:

stomach

Species:

human

Research Area:

Cancer Research/Cell Biology

Gastroenterology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	DS-135	1e5	88%	71%	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SF	DS-135	5e5	86-88%	65-83%	Plasmid (general)	2 µg	100 µl	4D X-Unit
V	B-023	1e6	70-76%	54-70%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

Peptide-Mediated Disruption of NFB/NRF Interaction Inhibits IL-8 Gene Activation by IL-1 or Helicobacter pylori

Categories:

Transfection

Authors:

Bartels M, Schweda AT, Dreikhausen U, Frank R, Resch K, Beil W, Nourbakhsh M

In:

J Immunol (2007) 179(11): 7605-13

Identification of a gastrin response element in the vesicular monoamine transporter type 2 promoter and requirement of 20S proteasome subunits for transcriptional activity