Ramos
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Burkitt's lymphoma, B lymphocyte
Cell Type:
B cell
Tissue Origin:
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SG
CA-137
2e6
51%
75-79%
Plasmid (general)
2 µg
100 µl
4D X-Unit
SG
CA-137
4e5
39-41%
69-71%
Plasmid (general)
400 ng
20 µl
4D X-Unit
V
O-006
2e6
20-34%
67-77%
Plasmid (general)
2 µg
100 µl
I/II/2b
SG
CA-137
5e5
20-30%
70-86%
siRNA
20 µl
96-well
SG
CM-138
5e5
20-30%
70-86%
siRNA
20 µl
96-well
Citations
High CD21 expression inhibits internalization of anti-CD19 antibodies and cytotoxicity of an anti-CD19-drug conjugate
Categories:
Transfection
Authors:
Ingle GS, Chan P, Elliott JM, Chang WS, Koeppen H, Stephan JP, Scales SJ
In:
Br J of Haematol (2008) 140(1): 46-58
The Immunomodulatory Benzodiazepine Bz-423 Inhibits B-Cell Proliferation by Targeting c-Myc Protein for Rapid and Specific Degradation
Categories:
Transfection
Authors:
Sundberg TB, Ney GM, Subramanian C, Opipari AW Jr and Glick GD
In:
Cancer Res (2006) 66(3): 1775-1782
Mechanism of B-cell receptor-induced phosphorylation and activation of phospholipase C-gamma2
Categories:
Transfection
Authors:
Kim YJ, Sekiya F, Poulin B, Bae YS and Rhee SG
In:
Mol Cell Biol (2004) 24(22): 9986-9999
Anti-IgM-induced down-regulation of nuclear Thy28 protein expression in Ramos B lymphoma cells
Categories:
Transfection
Authors:
Jiang XZ, Toyota H, Yoshimoto T, Takada E, Asakura H, Mizuguchi J
In:
Apoptosis (2003) 8(5): 509-19