cells

fibroblast cell line

Cell Type:

Fibroblast

Tissue Origin:

dermal

Species:

human

Research Area:

Dermatology/Tissue Engineering

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SE	FF-120	2e5	95% ±3	73% ±16	Plasmid (general)	0.4 µg	20 µl	4D X-Unit
SE	FF-120	8e5	95% ±3	60.3% ±4	Plasmid (general)	2 µg	100 µl	4D X-Unit
R	X-001	8e5	48-56%	74-78%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

The PP2A-Integrator-CDK9 axis fine-tunes transcription and can be targeted therapeutically in cancer

Categories:

Transfection, Cell Lines and Primary Cancer Cells

Authors:

Vervoort SJ, Welsh SA, Devlin JR, Barbieri E, Knight DA, Offley S, Bjelosevic S, Costacurta M, Todorovski I, Kearney CJ, Sandow JJ, Fan Z, Blyth B, McLeod V, Vissers JHA, Pavic K, Martin BP, Gregory G, Demosthenous E, Zethoven M, Kong IY, Hawkins ED, Hogg SJ, Kelly MJ, Newbold A, Simpson KJ, Kauko O, Harvey KF, Ohlmeyer M, Westermarck J, Gray N, Gardini A, Johnstone RW

In:

Cell (2021) 184(12): 3143-3162

Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins

Categories:

Transfection

Authors:

Kim S, Kim D, Cho SW, Kim J, Kim JS

In:

Genome Res (2014) 24(6): 1012-9

A systematic evaluation of integration free reprogramming methods for deriving clinically relevant patient specific induced pluripotent stem (iPS) cells

Categories:

Classical Media and Reagents, Serum-free and Speciality Media, Transfection

Authors:

Goh PA, Caxaria S, Casper C, Rosales C, Warner TT, Coffey PJ, Nathwani AC

In:

PLoS ONE (2013) 8(11): e81622

BJ

Transfection Information

Citations