COS-1
Export
african green kidney, SV40 transformed
Cell Type:
Fibroblast
Tissue Origin:
kidney
Species:
monkey
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
SF
DN-100
2e5
89%
84-88%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
SF
DN-100
1e6
94%
76-86%
Plasmid (general)
2 µg
100 µl
4D X-Unit
V
D-005
8e5
48-50%
56-72%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Selection of the alternative exon 1 from the cd5 gene down-regulates membrane level of the protein in B lymphocytes
Categories:
Transfection
Authors:
Garaud S, Le Dantec C, Berthou C, Lydyard PM, Youinou P, Renaudineau Y
In:
J Immunol (2008) 181(3): 2010-8
The C terminus of the synovial sarcoma-associated SSX proteins interacts with the LIM homeobox protein LHX4
Categories:
Transfection
Authors:
de Bruijn DR, van Dijk AH, Willemse MP, van Kessel AG
In:
Oncogene (2008) 27(5): 653-62
The differential interactions of peroxisome proliferator-activated receptor gamma ligands with Tyr473 is a physical basis for their unique biological activities.
Categories:
Bioassay
Authors:
Einstein M, Akiyama TE, Castriota GA, Wang CF, McKeever B, Mosley RT, Becker JW, Moller DE, Meinke PT, Wood HB, Berger JP.
In:
Mol Pharmacol (2008) 73(1): 62-74
Scaffold protein harmonin (USH1C) provides molecular links between Usher syndrome type 1 and type 2
Categories:
Transfection
Authors:
Reiners J, van Wijk E, Marker T, Zimmermann U, Jurgens K, Te Brinke H, Overlack N, Roepman R, Knipper M, Kremer H and Wolfrum U
In:
Hum Mol Genet (2005) 14(24): 3933-3943
Interaction of nephrocystin-4 and RPGRIP1 is disrupted by nephronophthisis or Leber congenital amaurosis-associated mutations
Categories:
Transfection
Authors:
Roepman R, Letteboer SJ, Arts HH, van Beersum SE, Lu X, Krieger E, Ferreira PA and Cremers FP
In:
Proc Natl Acad Sci USA (2005) 102(51): 18520-18525
Fusion of the SUMO/Sentrin-specific protease 1 gene SENP1 and the embryonic polarity-related mesoderm development gene MESDC2 in a patient with an infantile teratoma and a constitutional t(12;15)(q13;q25)
Categories:
Transfection
Authors:
Veltman IM, Vreede LA, Cheng J, Looijenga LH, Janssen B, Schoenmakers EF, Yeh ET and van Kessel AG
In:
Hum Mol Genet (2005) 14(14): 1955-1963