COS-1

african green kidney, SV40 transformed

Cell Type:
Fibroblast
Tissue Origin:
kidney
Species:
monkey
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

UltraCULTURE™ Medium is a complete all purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines.
The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution (Cat. No. 17-605) per 500 ml.

The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium does not contain L-glutamine.

UltraCULTURE™ Medium may be supplemented with Cryoprotective Medium (Cat. No. 12-132) to cryopreserve cells in a serum-free environment.

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF DN-100 2e5 89% 84-88% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF DN-100 1e6 94% 76-86% Plasmid (general) 2 µg 100 µl 4D X-Unit
V D-005 8e5 48-50% 56-72% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Garaud S, Le Dantec C, Berthou C, Lydyard PM, Youinou P, Renaudineau Y 
In:
J Immunol (2008) 181(3): 2010-8 
Categories:
Transfection 
Authors:
de Bruijn DR, van Dijk AH, Willemse MP, van Kessel AG 
In:
Oncogene (2008) 27(5): 653-62 
Categories:
Bioassay  
Authors:
Einstein M, Akiyama TE, Castriota GA, Wang CF, McKeever B, Mosley RT, Becker JW, Moller DE, Meinke PT, Wood HB, Berger JP. 
In:
Mol Pharmacol (2008) 73(1): 62-74 
Categories:
Transfection 
Authors:
Reiners J, van Wijk E, Marker T, Zimmermann U, Jurgens K, Te Brinke H, Overlack N, Roepman R, Knipper M, Kremer H and Wolfrum U 
In:
Hum Mol Genet (2005) 14(24): 3933-3943 
Categories:
Transfection 
Authors:
Roepman R, Letteboer SJ, Arts HH, van Beersum SE, Lu X, Krieger E, Ferreira PA and Cremers FP 
In:
Proc Natl Acad Sci USA (2005) 102(51): 18520-18525 
Categories:
Transfection 
Authors:
Veltman IM, Vreede LA, Cheng J, Looijenga LH, Janssen B, Schoenmakers EF, Yeh ET and van Kessel AG 
In:
Hum Mol Genet (2005) 14(14): 1955-1963