bone marrow; established from a long-term bone marrow culture of BDF1 mice; FDCP = factor dependent cell-Paterson

Cell Type:
Leukocyte (unspec.)
Tissue Origin:
bone marrow
Research Area:
Immunotherapy / Hematology
Cell Characteristics:

Recommended Media

Iscove's Modified Dulbecco's Medium (IMDM) is a modified DME containing high glucose (4,500 mg/L), sodium pyruvate, additional amino acids, HEPES buffer (in liquid form), selenium and other components. IMDM is suitable for fast growing cells and can support the growth of a wide variety of mammalian cells.

Storage = 2ºC to 8ºC

12-722 with L-glutamine
12-726 without L-glutamine
12-915 with L-glutamine and screened to support hybridoma growth

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
R T-001 1e6 40% 63% Plasmid (general) 2 µg 100 µl I/II/2b
L T-020 2e6 81% 91% Plasmid (general) 0.5 µg 100 µl I/II/2b
L D-017 2e6 93% 64% Plasmid (general) 0.5 µg 100 µl I/II/2b