cells

bone marrow; established from a long-term bone marrow culture of BDF1 mice; FDCP = factor dependent cell-Paterson

Cell Type:

Leukocyte (unspec.)

Tissue Origin:

bone marrow

Species:

mouse

Research Area:

Immunotherapy / Hematology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
R	T-001	1e6	40%	63%	Plasmid (general)	2 µg	100 µl	I/II/2b
L	T-020	2e6	81%	91%	Plasmid (general)	0.5 µg	100 µl	I/II/2b
L	D-017	2e6	93%	64%	Plasmid (general)	0.5 µg	100 µl	I/II/2b

FDCP-Mix

Transfection Information