cells

Following liver injury, hepatic stellate cells undergo 'activation'.This results in a transition from quiescent vitamin-A-rich HSCs into proliferative, fibrogenic, contractile myofibroblasts.

Cell Type:

Myofibroblast

Tissue Origin:

liver

Species:

human

Research Area:

Cancer Research/Cell Biology

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	Fibroblast, basic	P-024	8e5	57%	good	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

Overexpression of bcl-2 by activated human hepatic stellate cells: resistance to apoptosis as a mechanism of progressive hepatic fibrogenesis in humans

Categories:

Transfection

Authors:

Novo E, Marra F, Zamara E, Valfre di Bonzo L, Monitillo L, Cannito S, Petrai I, Mazzocca A, Bonacchi A, Defranco R, Colombatto S, Autelli R, Pinzani M and Parola M

In:

Gut (2006) 55(8): 1174-82

Myofibroblast, human hepatic

Transfection Information

Citations