T cell hybridoma

Cell Type:
T Cell
Tissue Origin:
Research Area:
Immunotherapy / Hematology
Cell Characteristics:

Recommended Media

UltraDOMA-PF Protein-free Hydridoma Media are formulations designed for use with hybridoma cell lines of murine, human, and chimeric origin. UltraDOMA-PF Media are completely defined media and do not contain peptides or tissue extracts.
The use of UltraDOMA-PF Media significantly simplifies downstream processing since all proteins present in a given cell culture supernatant are produced by the cells. 

L-glutamine and HEPES buffer are included in the formulation.

Storage = 2ºC  to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V T-016 2e6 30% 50-60% Plasmid (general) 2 µg 100 µl I/II/2b
V T-001 2e6 30% 50-60% Plasmid (general) 2 µg 100 µl I/II/2b
SF CA-137 1e6 82.7% 60% Plasmid (general) 400 ng 20 µl 4D X-Unit
SF CM-138 1e6 77.3% 55% Plasmid (general) 400 ng 20 µl 4D X-Unit
SF CM-150 1e6 76% 45.7% Plasmid (general) 400 ng 20 µl 4D X-Unit
SF DS-138 1e6 83% 48.8% Plasmid (general) 400 ng 20 µl 4D X-Unit