SK-BR-3

mammary gland, adenocarcinoma

Cell Type:
Mammary Epith.
Tissue Origin:
breast
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF EO-117 2e5 63-70% 60-70% Plasmid (general) 0.8 µg 20 µl 4D X-Unit
SF EO-117 1e6 60-70% 60-70% Plasmid (general) 4 µg 100 µl 4D X-Unit
C E-009 5e5 50% 90-95% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Endo Y, Deonauth K, Prahalad P, Hoxter B, Zhu Y, Byers SW 
In:
PLoS ONE (2008) 3(7): e2714 
Categories:
Transfection 
Authors:
Cosgrave N, Hill AD, Young LS 
In:
J Mol Endocrinol (2006) 37(3): 377-90 
Categories:
Transfection 
Authors:
Lim KT, Cosgrave N, Hill AD, Young LS 
In:
Breast Cancer Res (2006) 8(3): R33