cells

Gastrointestinal Stromal Tumor line that expresses an activating KIT mutation. Tuveson et al. (2001) Oncogene 20(36):5054-8

Cell Type:

Stromal cell

Tissue Origin:

intestine

Species:

human

Research Area:

Cancer Research/Cell Biology

Dermatology/Tissue Engineering

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	V	T-020	1e6	50%	85%	Plasmid (general)	2 µg	100 µl	I/II/2b

Citations

Histone H2AX Is a Mediator of Gastrointestinal Stromal Tumor Cell Apoptosis following Treatment with Imatinib Mesylate

Categories:

Transfection

Authors:

Liu Y, Tseng M, Perdreau SA, Rossi F, Antonescu C, Besmer P, Fletcher JA, Duensing S, Duensing A

In:

Cancer Res (2007) 67(6): 2685-92

GIST882

Transfection Information

Citations