BA/F3

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pro B cell

Cell Type:
B cell
Tissue Origin:
Species:
mouse
Research Area:
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SG CM-147 4e5 77-78% 70% Plasmid (general) 400 ng 20 µl 4D X-Unit
SG CM-147 2e6 80% 59% Plasmid (general) 2 µg 100 µl 4D X-Unit
V X-001 2e6 82-94% 70-88% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (13)

Novel MEK1 mutation identified by mutational analysis of epidermal growth factor receptor signaling pathway genes in lung adenocarcinoma
Categories:
Transfection 
Authors:
Marks JL, Gong Y, Chitale D, Golas B, McLellan MD, Kasai Y, Ding L, Mardis ER, Wilson RK, Solit D, Levine R, Michel K, Thomas RK, Rusch VW, Ladanyi M, Pao W 
In:
Cell Res (2008) 68(14): 5524-8 
IL-3 induced c-Fos activation is modulated by Gab2-calcineurin interaction
Categories:
Transfection 
Authors:
Pyarajan S, Matejovic G, Pratt JC, Baksh S, Burakoff SJ 
In:
J Biol Chem (2008) epub: online 
Tumor Cell Killing Mechanisms of Epidermal Growth Factor Receptor (EGFR) Antibodies Are Not Affected by Lung Cancer-Associated EGFR Kinase Mutations
Categories:
Transfection 
Authors:
Peipp M, Schneider-Merck T, Dechant M, Beyer T, Lammerts van Bueren JJ, Bleeker WK, Parren PW, van de Winkel JG, Valerius T 
In:
J Immunol (2008) 180(6): 4338-45 
Rac1 and a GTPase-activating protein, MgcRacGAP, are required for nuclear translocation of STAT transcription factors
Categories:
Transfection 
Authors:
Kawashima T, Bao YC, Nomura Y, Moon Y, Tonozuka Y, Minoshima Y, Hatori T, Tsuchiya A, Kiyono M, Nosaka T, Nakajima H, Williams DA, Kitamura T 
In:
J Cell Biol (2006) 175(6): 937-46 
Novel D761Y and Common Secondary T790M Mutations in Epidermal Growth Factor Receptor-Mutant Lung Adenocarcinomas with Acquired Resistance to Kinase Inhibitors
Categories:
Transfection 
Authors:
Balak MN, Gong Y, Riely GJ, Somwar R, Li AR, Zakowski MF, Chiang A, Yang G, Ouerfelli O, Kris MG, Ladanyi M, Miller VA, Pao W 
In:
Clin Cancer Res (2006) 12(21): 6494-6501 
Cotreatment with Vorinostat (Suberoylanilide Hydroxamic Acid) Enhances Activity of Dasatinib (BMS-354825) against Imatinib Mesylate-Sensitive or Imatinib Mesylate-Resistant Chronic Myelogenous Leukemia Cells
Categories:
Transfection 
Authors:
Fiskus W, Pranpat M, Balasis M, Bali P, Estrella V, Kumaraswamy S, Rao R, Rocha K, Herger B, Lee F, Richon V, Bhalla K 
In:
Clin Cancer Res (2006) 12(19): 5869-78 
FOG-1 represses GATA-1-dependent FcRI -chain transcription: transcriptional mechanism of mast cell-specific gene expression in mice
Categories:
Transfection 
Authors:
Maeda K, Nishiyama C, Tokura T, Nakano H, Kanada S, Nishiyama M, Okumura K, Ogawa H 
In:
Blood (2006) 108(1): 262-9 
Combined effects of novel tyrosine kinase inhibitor AMN107 and histone deacetylase inhibitor LBH589 against Bcr-Abl expressing human leukemia cells
Categories:
Transfection 
Authors:
Fiskus W, Pranpat M, Bali P, Balasis M, Kumaraswamy S, Boyapalle S, Rocha K, Wu J, Giles F, Manley PW, Atadja P, Bhalla K 
In:
Blood (2006) 108(2): 645-52 
Sumoylation of Daxx Regulates IFN-Induced Growth Suppression of B Lymphocytes and the Hormone Receptor-Mediated Transactivation
Categories:
Transfection 
Authors:
Muromoto R, Ishida M, Sugiyama K, Sekine Y, Oritani K, Shimoda K, Matsuda T 
In:
J Immunol (2006) 177(2): 1160-70 
Constitutively activated FLT3 phosphorylates BAD partially through Pim-1
Categories:
Transfection 
Authors:
Kim KT, Levis M, Small D 
In:
Br J of Haematol (2006) 134(5): 500-509 
G-CSF induced reactive oxygen species involves Lyn-PI 3-kinase-Akt and contributes to myeloid cell growth
Categories:
Transfection 
Authors:
Zhu QS, Xia L, Mills GB, Lowell CA, Touw IP and Corey SJ 
In:
Blood (2006) 107(5): 1847-56 
An alternative inhibitor overcomes resistance caused by a mutation of the epidermal growth factor receptor
Categories:
Transfection 
Authors:
Kobayashi S, Ji H, Yuza Y, Meyerson M, Wong KK, Tenen DG and Halmos B 
In:
Cancer Res (2005) 65(16): 7096-7101 
CD200R family members represent novel DAP12-associated activating receptors on basophils and mast cells
Categories:
Transfection 
Authors:
Voehringer D, Rosen DB, Lanier LL and Locksley RM 
In:
J Biol Chem (2004) 279(52): 54117-54123 
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