HCT 116

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colorectal carcinoma cell line, epithelial

Cell Type:
GI Tract Epith.
Tissue Origin:
colon
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

McCoy's 5A Medium

McCoy's 5A Medium was developed in 1956 to be formulated for the nutritional requirements of Walker 256 carcinoma. Its unique components include asparagine, 3X normal glucose, amino acids (all Lisomers) and increased folic acid. It is a general purpose medium for primary and established cell lines.

12-168 With L-glutamine and 25 mM HEPES 
12-688 With L-glutamine

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V D-032 1e6 74-82% 66-86% Plasmid (general) 2 µg 100 µl I/II/2b
SE EN-113 1e6 94% 59-63% Plasmid (general) µg 100 µl 4D X-Unit
SE EN-113 2e5 93% 62% Plasmid (general) 400 ng 20 µl 4D X-Unit
SE DS-138 2e5 80% decent Plasmid (general) 400 ng 20 µl 96-well
SE EH-100 2e5 70-80% 65-75% Plasmid (general) 0.4 µg 20 µl 96-well
SE FP-100 2e5 70-80% 65-75% Plasmid (general) 0.4 µg 20 µl 96-well

Citations (13)

Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair
Categories:
Transfection 
Authors:
Wienert B, Nguyen DN, Guenther A, Feng SJ, Locke MN, Wyman SK, Shin J, Kazane KR, Gregory GL, Carter MAM, Wright F, Conklin BR, Marson A, Richardson CD, Corn JE 
In:
Nat Commun. (2020) 11(1): 2109 
High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs.
Categories:
Transfection 
Authors:
Duda K, Lonowski LA, Kofoed-Nielsen M, Ibarra A, Delay CM, Kang Q, Yang Z, Pruett-Miller SM, Bennett EP3, Wandall HH, Davis GD, Hansen SH, Frödin M. 
In:
Nucleic Acids Res (2014) 42: 1-16 
Involvement of protective autophagy in TRAIL-resistance of apoptosis defective tumor cells
Categories:
Transfection 
Authors:
Han J, Hou W, Goldstein LA, Lu C, Stolz DB, Yin XM, Rabinowich H 
In:
J Biol Chem (2008) 283(28): 19665-77 
Epigenetic silencing of microRNA-34b/c and B-cell translocation gene 4 is associated with CpG island methylation in colorectal cancer
Categories:
Transfection 
Authors:
Toyota M, Suzuki H, Sasaki Y, Maruyama R, Imai K, Shinomura Y, Tokino T 
In:
Cancer Res (2008) 68(11): 4123-32 
The mitochondrial uncoupling protein-2 promotes chemoresistance in cancer cells
Categories:
Transfection 
Authors:
Derdak Z, Mark NM, Beldi G, Robson SC, Wands JR, Baffy G 
In:
Cancer Res (2008) 68(8): 2813-9 
Serial analysis of chromatin occupancy identifies beta-catenin target genes in colorectal carcinoma cells
Categories:
Transfection 
Authors:
Yochum GS, McWeeney S, Rajaraman V, Cleland R, Peters S, Goodman RH 
In:
Proc Natl Acad Sci USA (2007) 104(9): 3324-9 
An antisense transcript induced by WNT/ beta-catenin signaling decreases E2F4
Categories:
Transfection 
Authors:
Yochum GS, Cleland R, McWeeney S, Goodman RH 
In:
J Biol Chem (2007) 282(2): 871-8 
Inhibition of centrosome protein assembly leads to p53-dependent exit from the cell cycle
Categories:
Transfection 
Authors:
Srsen V, Gnadt N, Dammermann A, Merdes A 
In:
J Cell Biol (2006) 174(5): 625-30 
Gli1, down-regulated in colorectal cancers, inhibits proliferation of colon cancer cells involving Wnt signalling activation
Categories:
Transfection 
Authors:
Akiyoshi T, Nakamura M, Koga K, Nakashima H, Yao T, Tsuneyoshi M, Tanaka M and Katano M 
In:
Gut (2006) 55(7): 991-9 
Interrelated roles for Mcl-1 and Bim in regulation of TRAIL-mediated mitochondrial apoptosis
Categories:
Transfection 
Authors:
Han J, Goldstein LA, Gastman BR, Rabinowich H 
In:
J Biol Chem (2006) 281(15): 10153-63 
Elevated expression of C10orf3 (chromosome 10 open reading frame 3) is involved in the growth of human colon tumor
Categories:
Transfection 
Authors:
Sakai M, Shimokawa T, Kobayashi T, Matsushima S, Yamada Y, Nakamura Y and Furukawa Y 
In:
Oncogene (2006) 25(3): 480-6 
Disruption of MCL-1/BIM complex in granzyme B-mediated mitochondrial apoptosis
Categories:
Transfection 
Authors:
Han J, Goldstein LA, Gastman BR, Rabinovitz A and Rabinowich H 
In:
J Biol Chem (2005) 280(16): 16383-16392 
p53CSV, a novel p53-inducible gene involved in the p53-dependent cell-survival pathway
Categories:
Transfection 
Authors:
Park WR and Nakamura Y 
In:
Cancer Res (2005) 65(4): 1197-1206 
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