HCT 116

colorectal carcinoma cell line, epithelial

Cell Type:
GI Tract Epith.
Tissue Origin:
colon
Species:
human
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

McCoy's 5A Medium was developed in 1956 to be formulated for the nutritional requirements of Walker 256 carcinoma. Its unique components include asparagine, 3X normal glucose, amino acids (all Lisomers) and increased folic acid. It is a general purpose medium for primary and established cell lines.

12-168 With L-glutamine and 25 mM HEPES 
12-688 With L-glutamine

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V D-032 1e6 74-82% 66-86% Plasmid (general) 2 µg 100 µl I/II/2b
SE EN-113 1e6 94% 59-63% Plasmid (general) µg 100 µl 4D X-Unit
SE EN-113 2e5 93% 62% Plasmid (general) 400 ng 20 µl 4D X-Unit
SE DS-138 2e5 80% decent Plasmid (general) 400 ng 20 µl Shuttle
SE EH-100 2e5 70-80% 65-75% Plasmid (general) 0.4 µg 20 µl Shuttle
SE FP-100 2e5 70-80% 65-75% Plasmid (general) 0.4 µg 20 µl Shuttle

Citations (12)

Categories:
Transfection 
Authors:
Duda K, Lonowski LA, Kofoed-Nielsen M, Ibarra A, Delay CM, Kang Q, Yang Z, Pruett-Miller SM, Bennett EP3, Wandall HH, Davis GD, Hansen SH, Frödin M. 
In:
Nucleic Acids Res (2014) 42: 1-16 
Categories:
Transfection 
Authors:
Han J, Hou W, Goldstein LA, Lu C, Stolz DB, Yin XM, Rabinowich H 
In:
J Biol Chem (2008) 283(28): 19665-77 
Categories:
Transfection 
Authors:
Toyota M, Suzuki H, Sasaki Y, Maruyama R, Imai K, Shinomura Y, Tokino T 
In:
Cancer Res (2008) 68(11): 4123-32 
Categories:
Transfection 
Authors:
Derdak Z, Mark NM, Beldi G, Robson SC, Wands JR, Baffy G 
In:
Cancer Res (2008) 68(8): 2813-9 
Categories:
Transfection 
Authors:
Yochum GS, McWeeney S, Rajaraman V, Cleland R, Peters S, Goodman RH 
In:
Proc Natl Acad Sci USA (2007) 104(9): 3324-9 
Categories:
Transfection 
Authors:
Yochum GS, Cleland R, McWeeney S, Goodman RH 
In:
J Biol Chem (2007) 282(2): 871-8 
Categories:
Transfection 
Authors:
Srsen V, Gnadt N, Dammermann A, Merdes A 
In:
J Cell Biol (2006) 174(5): 625-30 
Categories:
Transfection 
Authors:
Akiyoshi T, Nakamura M, Koga K, Nakashima H, Yao T, Tsuneyoshi M, Tanaka M and Katano M 
In:
Gut (2006) 55(7): 991-9 
Categories:
Transfection 
Authors:
Han J, Goldstein LA, Gastman BR, Rabinowich H 
In:
J Biol Chem (2006) 281(15): 10153-63 
Categories:
Transfection 
Authors:
Sakai M, Shimokawa T, Kobayashi T, Matsushima S, Yamada Y, Nakamura Y and Furukawa Y 
In:
Oncogene (2006) 25(3): 480-6 
Categories:
Transfection 
Authors:
Han J, Goldstein LA, Gastman BR, Rabinovitz A and Rabinowich H 
In:
J Biol Chem (2005) 280(16): 16383-16392 
Categories:
Transfection 
Authors:
Park WR and Nakamura Y 
In:
Cancer Res (2005) 65(4): 1197-1206 
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