Epithelial, airway, human

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Cells isolated from lining of human airway.

Cell Type:
Respiratory Tract Epith.
Tissue Origin:
lung
Species:
human
Research Area:
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Epithelial, basic S-005 2e6 80% 50% Plasmid (general) 5 µg 100 µl I/II/2b

Citations (15)

A functional genetic toolbox for human tissue-derived organoids
Categories:
3D Cell Culture , Transfection 
Authors:
Dawei Sun , Lewis Evans , Francesca Perrone , Vanesa Sokleva , Kyungtae Lim , Saba Rezakhani , Matthias Lutolf , Matthias Zilbauer , Emma L Rawlins  
In:
eLife (2021) 10: e67886 
Th17 Cytokines Disrupt the Airway Mucosal Barrier in Chronic Rhinosinusitis.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ramezanpour M, Moraitis S, Smith JL, Wormald PJ, Vreugde S 
In:
Mediators Inflamm (2016) 2016:9798206: doi: 10.1155/2016/9798206 
CX3CR1 Is Expressed in Differentiated Human Ciliated Airway Cells and Co-Localizes With Respiratory Syncytial Virus on Cilia in a G Protein-Dependent Manner 
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Kwang-Il Jeong  1 , Peter A Piepenhagen  2 , Michael Kishko  1 , Joshua M DiNapoli  1 , Rachel P Groppo  1 , Linong Zhang  1 , Jeffrey Almond  1 , Harry Kleanthous  1 , Simon Delagrave  1 , Mark Parrington  1              
In:
PLoS ONE (2015) 10: 6 
Monocyte interaction accelerates HCl-induced lung epithelial remodeling.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chen Q, Luo AA, Qiu H, Han B, Ko BH, Slutsky AS, Zhang H 
In:
BMC Pulm Med (2014) 14(135): 1471-79 
Bronchial epithelial cells are rendered insensitive to glucocorticoid transactivation by transforming growth factor-ß1.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Keenan CR, Mok JS, Harris T, Xia Y, Salem S, Stewart AG 
In:
Respir Res (2014) 15(55): 1-15 
Effect of oxidative stress on respiratory epithelium from children with Down syndrome
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bruijn M, Lutter R, Eldering E, Bos AP, van Woensel JB. 
In:
Eur Respir J (2013) 42(4): 1037-45 
Systems approaches to modeling chronic mucosal inflammation.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kalita M, Tian B, Gao B, Choudhary S, Wood TG, Carmical JR, Boldogh I, Mitra S, Minna JD, Brasier AR. 
In:
Biomed Res Int. (2013) 2013: 17 
The nitrated fatty acid 10-nitro-oleate attenuates allergic airway disease.
Categories:
Primary Cells and Media 
Authors:
Reddy AT, Lakshmi SP, Dornadula S, Pinni S, Rampa DR, Reddy RC. 
In:
J Immunol (2013) 191(5): 2053-63 
Rhinovirus-induced MMP-9 expression is dependent on Fra-1, which is modulated by formoterol and dexamethasone.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Tacon CE, Newton R, Proud D, Leigh R. 
In:
J Immunol (2012) 88(9): 4621-30 
Resistin-like molecule-ß is a human airway remodelling mediator.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fang C, Meng Q, Wu H, Eid G, Zhang G, Zhang X, Yang S, Huang K, Lee TH, Corrigan CJ, Ying S. 
In:
Eur Respir J (2012) 39(2): 458-466 
Regulation of normal and cystic fibrosis airway epithelial repair processes by TNF-a after injury.
Categories:
 
Authors:
Maillé E, Trinh NT, Privé A, Bilodeau C, Bissonnette E, Grandvaux N, Brochiero E. 
In:
Am J Physiol Lung Cell Mol Physiol (2011) 301(6): L945-55 
Inner dynein arm defects causing primary ciliary dyskinesia: repeat testing required.
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
C. O’Callaghan, A. Rutman, G.M. Williams and R.A. Hirst 
In:
Eur Respir J (2011) 38(3): 603-607 
Role of NF-kappaB and PPAR-gamma in lung inflammation induced by monocyte-derived microparticles
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Neri T, Armani C, Pegoli A, Cordazzo C, Carmazzi Y, Brunelleschi S, Bardelli C, Breschi MC, Paggiaro P, Celi A. 
In:
Eur Respir J (2011) 37(6): 1494-502 
Cell-permeable peptide DEPDC1-ZNF224 interferes with transcriptional repression and oncogenicity in bladder cancer cells.
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Harada Y, Kanehira M, Fujisawa Y, Takata R, Shuin T, Miki T, Fujioka T, Nakamura Y, Katagiri T. 
In:
Cancer Res (2010) 70(14): 5829-39 
Syk Is Downstream of Intercellular Adhesion Molecule-1 and Mediates Human Rhinovirus Activation of p38 MAPK in Airway Epithelial Cells
Categories:
Transfection 
Authors:
Wang X, Lau C, Wiehler S, Pow A, Mazzulli T, Gutierrez C, Proud D, Chow CW 
In:
J Immunol (2006) 177(10): 6859-70 
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