Epithelial, airway, human

Cells isolated from lining of human airway.

Cell Type:
Respiratory Tract Epith.
Tissue Origin:
lung
Species:
human
Research Area:
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Epithelial, basic S-005 2e6 80% 50% Plasmid (general) 5 µg 100 µl I/II/2b

Citations (14)

Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Ramezanpour M, Moraitis S, Smith JL, Wormald PJ, Vreugde S 
In:
Mediators Inflamm (2016) 2016:9798206: doi: 10.1155/2016/9798206 
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Kwang-Il Jeong  1 , Peter A Piepenhagen  2 , Michael Kishko  1 , Joshua M DiNapoli  1 , Rachel P Groppo  1 , Linong Zhang  1 , Jeffrey Almond  1 , Harry Kleanthous  1 , Simon Delagrave  1 , Mark Parrington  1              
In:
PLoS ONE (2015) 10: 6 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Chen Q, Luo AA, Qiu H, Han B, Ko BH, Slutsky AS, Zhang H 
In:
BMC Pulm Med (2014) 14(135): 1471-79 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Keenan CR, Mok JS, Harris T, Xia Y, Salem S, Stewart AG 
In:
Respir Res (2014) 15(55): 1-15 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Bruijn M, Lutter R, Eldering E, Bos AP, van Woensel JB. 
In:
Eur Respir J (2013) 42(4): 1037-45 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kalita M, Tian B, Gao B, Choudhary S, Wood TG, Carmical JR, Boldogh I, Mitra S, Minna JD, Brasier AR. 
In:
Biomed Res Int. (2013) 2013: 17 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Reddy AT, Lakshmi SP, Dornadula S, Pinni S, Rampa DR, Reddy RC. 
In:
J Immunol (2013) 191(5): 2053-63 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Tacon CE, Newton R, Proud D, Leigh R. 
In:
J Immunol (2012) 88(9): 4621-30 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fang C, Meng Q, Wu H, Eid G, Zhang G, Zhang X, Yang S, Huang K, Lee TH, Corrigan CJ, Ying S. 
In:
Eur Respir J (2012) 39(2): 458-466 
Categories:
 
Authors:
Maillé E, Trinh NT, Privé A, Bilodeau C, Bissonnette E, Grandvaux N, Brochiero E. 
In:
Am J Physiol Lung Cell Mol Physiol (2011) 301(6): L945-55 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
C. O’Callaghan, A. Rutman, G.M. Williams and R.A. Hirst 
In:
Eur Respir J (2011) 38(3): 603-607 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Neri T, Armani C, Pegoli A, Cordazzo C, Carmazzi Y, Brunelleschi S, Bardelli C, Breschi MC, Paggiaro P, Celi A. 
In:
Eur Respir J (2011) 37(6): 1494-502 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Harada Y, Kanehira M, Fujisawa Y, Takata R, Shuin T, Miki T, Fujioka T, Nakamura Y, Katagiri T. 
In:
Cancer Res (2010) 70(14): 5829-39 
Categories:
Transfection 
Authors:
Wang X, Lau C, Wiehler S, Pow A, Mazzulli T, Gutierrez C, Proud D, Chow CW 
In:
J Immunol (2006) 177(10): 6859-70 
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