EAhy926
Export
endothelial cell line, derived from fusion of HUVEC with A549
Cell Type:
Umbilical Vein Endo.
Tissue Origin:
Species:
human
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
V
A-023
7e5
55%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
T-001
7e5
58%
Plasmid (general)
2 µg
100 µl
I/II/2b
V
O-017
7e5
59%
Plasmid (general)
2 µg
100 µl
I/II/2b
SE
DS-120
2e5
93.1%
85.6%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
SE
EW-113
2e5
94.6%
87.9%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
SE
EO-100
2e5
94.8%
89.3%
Plasmid (general)
0.4 µg
20 µl
4D X-Unit
Citations
Nitric oxide activation of peroxisome proliferator-activated receptor gamma through a p38 MAPK signaling pathway
Categories:
Transfection
Authors:
Ptasinska A, Wang S, Zhang J, Wesley RA, Danner RL
In:
FASEB J (2007) 21(3): 950-61