32D

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lymphoblast, established from long-term bone marrow cultures of C3H/HeJ mice infected with the Friend murine leukemia virus. Morphology: mostly round cells growing singly or in small clusters in suspension, some cells are loosely adherent.

Cell Type:
Leukocyte (unspec.)
Tissue Origin:
bone marrow
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
V E-032 1e6 72-86% 56-66% Plasmid (general) 2 µg 100 µl I/II/2b
SF CV-137 2e6 61-77% 75-93% Plasmid (general) 2 µg 100 µl 4D X-Unit
SF CV-137 4e5 58-76% 74-88% Plasmid (general) 0.4 µg 20 µl 4D X-Unit

Citations

Survival signaling by C-RAF: mitochondrial ROS and Ca2+ are critical targets
Categories:
Transfection 
Authors:
Kuznetsov AV, Smigelskaite J, Doblander C, Janakiraman M, Hermann M, Wurm M, Scheidl S, Sucher R, Deutschmann A, Troppmair J 
In:
Mol Cell Biol (2008) 28(7): 2304-13 
Mechanism of All-Trans Retinoic Acid Effect on Tumor-Associated Myeloid-Derived Suppressor Cells
Categories:
Transfection 
Authors:
Nefedova Y, Fishman M, Sherman S, Wang X, Beg AA, Gabrilovich DI 
In:
Cancer Res (2007) 67(22): 11021-11028 
Transcription factor Gfi-1 induced by G-CSF is a negative regulator of CXCR4 in myeloid cells
Categories:
Transfection 
Authors:
De La Luz Sierra M, Gasperini P, McCormick PJ, Zhu J, Tosato G 
In:
Blood (2007) 110(7): 2276-85 
Activation mechanisms of STAT5 by oncogenic Flt3-ITD
Categories:
Transfection 
Authors:
Choudhary C, Brandts C, Schwable J, Tickenbrock L, Sargin B, Ueker A, Bohmer FD, Berdel WE, Muller-Tidow C, Serve H 
In:
Blood (2007) 110(1): 370-4 
Recruitment of insulin receptor substrate-1 and activation of NF-kappaB essential for midkine growth signaling through anaplastic lymphoma kinase
Categories:
Transfection 
Authors:
Kuo AH, Stoica GE, Riegel AT, Wellstein A 
In:
Oncogene (2007) 26(6): 859-69 

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