Toxoplasma gondii

Export

T. gondii is an obligate intracellular parasite and has very low host specificity. Its life cycle includes two phases: intestinal (or enteroepithelial) and extraintestinal.

Cell Type:
Toxoplasma
Tissue Origin:
Species:
unicellular
Research Area:
Parasitology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 FI-158 Plasmid (general) 20 µl 4D X-Unit

Citations (7)

GRA12 is a common virulence factor across Toxoplasma gondii strains and mouse subspecies
Categories:
Transfection, Protozoa 
Authors:
Francesca Torelli, Simon Butterworth, Eloise Lockyer, Ana N. Matias, Franziska Hildebrandt, Ok-Ryul Song, Jennifer Pearson-Farr & Moritz Treeck 
In:
Nat Commun. (2025) 16:  
Orthologs of Plasmodium ICM1 are dispensable for Ca2+ mobilization in Toxoplasma gondii
Categories:
Transfection, Protozoa 
Authors:
 Gabriel Cabral, Bingjian Ren, Hugo Bisio,  Dawson Otey,  Dominique Soldati-Favre,  Kevin M. Brown 
In:
(2024) :  
The SUN-like protein TgSLP1 is essential for nuclear division in the apicomplexan parasite Toxoplasma gondii
Categories:
Transfection, Protozoa 
Authors:
Mirjam Wagner, Yuan Song, Elena Jiménez-Ruiz  , Sonja Härtle, Markus Meissner 
In:
J Cell Sci (2023) 136: 21 
Toxoplasma ceramide synthases: Gene duplication, functional divergence, and roles in parasite fitness
Categories:
Transfection, Protozoa 
Authors:
Zisis Koutsogiannis, John G. Mina, Christin A. Albus, Matthijs A. Kol, Joost C. M. Holthuis, Ehmke Pohl, Paul W. Denny 
In:
FASEB J (2023) 37: 11 
Cellular barcoding of protozoan pathogens reveals the within-host population dynamics of Toxoplasma gondii host colonization
Categories:
Transfection, Protozoa 
Authors:
Ceire J Wincott, Gayathri Sritharan, Henry J Benns, Dana May, Carla Gilabert-Carbajo, Monique Bunyan, Aisling R Fairweather, Eduardo Alves, Ivan Andrew, Laurence Game, Eva-Maria Frickel, Calvin Tiengwe, Sarah E Ewald,  Matthew A Child 
In:
Cell Rep (2022) 2: 8 
Protein O-fucosyltransferase 2-mediated O-glycosylation of the adhesin MIC2 is dispensable for Toxoplasma gondii tachyzoite infection.
Categories:
Transfection, Protozoa 
Authors:
Khurana S, Coffey MJ, John A, Uboldi AD, Huynh MH, Stewart RJ, Carruthers V, Tonkin CJ, Goddard-Borger ED, Scott NE 
In:
J Biol Chem (2018) Epub:  
The CRISPR/Cas9 system sheds new lights on the biology of protozoan parasites.
Categories:
Transfection 
Authors:
Grzybek M1, Golonko A2, Górska A3, Szczepaniak K3, Strachecka A4, Lass A5, Lisowski P6,7. 
In:
Biotechnology Journal (2018) 102: 11 
+ Show All

Privacy | Legal | About Lonza

© Lonza. All rights reserved.