cells

Type II lung epithelial cells are cuboidal and occupy only 5% of surface area (type I are squamous and occupy 95%). They produce, secrete and recycle lung surfactant; and can be converted to type I cells to repair damaged epithelium.

Cell Type:

Respiratory Tract Epith.

Tissue Origin:

lung

Species:

human

Research Area:

Dermatology/Tissue Engineering

Cell Characteristics:

Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	Epithelial, basic	T-020	2e6	20-40%	50%	Plasmid (general)	4 µg	100 µl	I/II/2b

Citations

Pepsinogen C proteolytic processing of surfactant protein B

Categories:

Transfection

Authors:

Gerson KD, Foster CD, Zhang P, Zhang Z, Rosenblatt MM, Guttentag SH

In:

J Biol Chem (2008) 283(16): 10330-8

Developmental Regulation of Duox1 Expression and Function in Human Fetal Lung Epithelial Cells

Categories:

Transfection

Authors:

Fischer H, Gonzales LK, Kolla V, Schwarzer C, Miot F, Illek B, Ballard PL

In:

Am J Physiol Lung Cell Mol Physiol (2007) 292(6): L1506-14

Thyroid Transcription Factor in Differentiating Type II Cells: Regulation, Isoforms and Target Genes

Categories:

Transfection

Authors:

Kolla V, Gonzales LW, Gonzales J, Wang P, Angampalli S, Feinstein SI, Ballard PL

In:

Am J Respir Cell Mol Biol (2007) 36(2): 213-25

Epithelial, lung type II, human

Transfection Information

Citations