Epithelial, cornea, human
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Primary epithelial cell isolated from human cornea
Cell Type:
Eye Epith.
Tissue Origin:
eye
Species:
human
Research Area:
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
Epithelial, basic
T-023
2e6
40-50%
70%
Plasmid (general)
4 µg
100 µl
I/II/2b
Citations
Corneal Regeneration by Deep Anterior Lamellar Keratoplasty (DALK) Using Decellularized Corneal Matrix
Categories:
Primary Cells and Media
Authors:
Hashimoto Y, Funamoto S, Sasaki S, Negishi J, Honda T, Hattori S, Nam K, Kimura T, Mochizuki M, Kobayashi H, Kishida A.
In:
PLoS ONE (2015) 10(7): 1989
Pseudomonas aeruginosa-induced bleb-niche formation in epithelial cells is independent of actinomyosin contraction and enhanced by loss of cystic fibrosis transmembrane-conductance regulator osmoregulatory function
Categories:
Classical Media and Reagents, Serum-free and Speciality Media
Authors:
Jolly AL, Takawira D, Oke OO, Whiteside SA, Chang SW, Wen ER, Quach K, Evans DJ, Fleiszig SM
In:
mBio (2015) 6(2): e02533
Enzymes responsible for synthesis of corneal keratan sulfate glycosaminoglycans
Categories:
Transfection
Authors:
Kitayama K, Hayashida Y, Nishida K, Akama TO
In:
J Biol Chem (2007) 282(41): 30085-96
Urokinase receptor cleavage: a crucial step in fibroblast-to-myofibroblast differentiation
Categories:
Transfection
Authors:
Bernstein AM, Twining SS, Warejcka DJ, Tall E, Masur SK
In:
Mol Biol Cell (2007) 18(7): 2716-27
In vivo and in vitro expression of connexins in the human corneal epithelium
Categories:
Transfection
Authors:
Shurman DL, Glazewski L, Gumpert A, Zieske JD and Richard G
In:
Invest Ophthalmol Vis Sci (2005) 46(6): 1957-1965