cells

macrophage cell line

Cell Type:

Macrophage

Tissue Origin:

ascites

Species:

mouse

Research Area:

Immunotherapy / Hematology

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
SF	CM-139	2e5	42%	66%	mRNA	0.8 µg	20 µl	4D X-Unit
SF	CM-139	2e5	87%	92%	siRNA	0.6 µg	20 µl	4D X-Unit
SF	CM-139	1e6	74%	96%	siRNA	3 µg	100 µl	4D X-Unit

Citations

Identification of innate immunity genes and pathways using a comparative genomics approach

Categories:

Transfection

Authors:

Alper S, Laws R, Lackford B, Boyd WA, Dunlap P, Freedman JH, Schwartz DA

In:

Proc Natl Acad Sci USA (2008) 105(19): 7016-21

mRNA but not plasmid DNA is efficiently transfected in murine J774A.1 macrophages

Categories:

Transfection

Authors:

Van De Parre TJ, Martinet W, Schrijvers DM, Herman AG and De Meyer GR

In:

Biochem Biophys Res Commun (2005) 327(1): 356-360