Trypanosoma cruzi

Causative agent of American trypanosomiasis (Chagas disease). In humans found as both an intracellular form, the amastigote, and as a trypomastigote form in the blood. Chagas disease affects primarily the nervous system and heart.

Cell Type:
Trypanosoma
Tissue Origin:
Species:
unicellular
Research Area:
Parasitology
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
P3 EH-100 100 µl 4D X-Unit

Citations

Categories:
Transfection, Protozoa 
Authors:
Thaise Lara Teixeira, Miguel Angel Chiurillo, Noelia Lander, Cassiano Costa Rodrigues, Thiago Souza Onofre, Éden Ramalho Ferreira, Camila Miyagui Yonamine, Júlia de Gouveia Santos, Renato Arruda Mortara, Claudio Vieira da Silva, José Franco da Silveira 
In:
(2022) 12:  
Categories:
Transfection, Protozoa 
Authors:
Esteban M. Cordero, Cristian Cortez, Nobuko Yoshida, José Franco da Silveira 
In:
Scientific Reports (2019) :  
Categories:
Transfection 
Authors:
Grzybek M1, Golonko A2, Górska A3, Szczepaniak K3, Strachecka A4, Lass A5, Lisowski P6,7. 
In:
Biotechnology Journal (2018) 102: 11 
Categories:
Transfection 
Authors:
Martin C. Taylor, Michael D. Lewis, Amanda Fortes Francisco, Shane R. Wilkinson, John M. Kelly 
In:
Other (2015) 9(4): e0003707 
Categories:
 
Authors:
Duo Peng, Samarchith P. Kurup, Phil Y. Yao, Todd A. Minning, Rick L. Tarletona 
In:
mBio (2014) 6 (1): e02097-14 
Categories:
Transfection 
Authors:
Coustou V, Guegan F, Plazolles N, Baltz T. 
In:
PLoS Biol (2010) 4(3): 618