5838 Ewing's
Export
human sarcoma
Cell Type:
Bone
Tissue Origin:
Species:
human
Cell Characteristics:
Adherent
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
R
O-017
2e6
77%
90%
Plasmid (general)
2 µg
100 µl
I/II/2b
R
T-001
2e6
71%
88%
Plasmid (general)
2 µg
100 µl
I/II/2b
R
G-016
2e6
61%
92%
Plasmid (general)
2 µg
100 µl
I/II/2b
Citations
Cancer translocations in human cells induced by zinc finger and TALE nucleases.
Categories:
Transfection
Authors:
Piganeau M, Ghezraoui H, De Cian A, Guittat L, Tomishima M, Perrouault L, René O, Katibah GE, Zhang L, Holmes MC, Doyon Y, Concordet JP, Giovannangeli C, Jasin M, Brunet E.
In:
Genome Res (2013) 23(7): 1182-93