Bone marrow, mouse

Cells isolated from murine bone marrow.

Cell Type:
Blood/Bone Marrow SC
Tissue Origin:
bone marrow
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Stem Cells
Cell Characteristics:
Suspension

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Macrophage, mouse T-030 3e6 10% 59% Plasmid (general) 1 µg 100 µl I/II/2b

Citations (8)

Categories:
Classical Media and Reagents, Transfection 
Authors:
Maria José Pino-Barrio , Yari Giménez, Mariela Villanueva , Marcus Hildenbeutel , Rebeca Sánchez-Dominguez , Sandra Rodríguez-Perales , Roser Pujol , Jordi Surrallés , Paula Río  , Toni Cathomen , Claudio Mussolino , Juan Antonio Bueren , Susana Navarro 
In:
Scientific Reports (2020) 10(1): 6997 
Categories:
Transfection 
Authors:
Ngoc Tung Tran , Thomas Sommermann , Robin Graf , Janine Trombke , Jenniffer Pempe , Kerstin Petsch , Ralf Kühn , Klaus Rajewsky , Van Trung Chu  
In:
Cell Rep (2019) 28(13): 3510-3522 
Categories:
Primary Cells and Media 
Authors:
Kaneda MM, Cappello P, Nguyen AV, Ralainirina N, Hardamon CR, Foubert P, Schmid MC, Sun P, Mose E, Bouvet M, Lowy AM, Valasek MA, Sasik R, Novelli F, Hirsch E, Varner JA 
In:
Cancers (2016) 6(8): 870-885 
Categories:
Transfection 
Authors:
Cheng P, Nefedova Y, Corzo CA, Gabrilovich DI 
In:
Blood (2007) 109(2): 507-15 
Categories:
Transfection 
Authors:
Takizawa H, Kubo-Akashi C, Nobuhisa I, Kwon SM, Iseki M, Taga T, Takatsu K and Takaki S 
In:
Blood (2006) 107(7): 2968-75 
Categories:
Transfection 
Authors:
Lucas M, Zhang X, Prasanna V and Mosser DM 
In:
J Immunol (2005) 175(1): 469-477 
Categories:
Transfection 
Authors:
Lum JJ, Bauer DE, Kong M, Harris MH, Li C, Lindsten T and Thompson CB 
In:
Cell (2005) 120(2): 237-248 
Categories:
Transfection 
Authors:
Lakshmipathy U, Pelacho B, Sudo K, Linehan JL, Coucouvanis E, Kaufman DS and Verfaillie CM 
In:
Stem Cells (2004) 22(4): 531-543 
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