Hep G2

Human hepatocellular carcinoma cell line

Cell Type:
Liver Epith.
Tissue Origin:
liver
Species:
human
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Toxicology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF

EH-100

2e5 72.1% ±5 92% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF

EH-100

1e6 68.9% ±2 92% Plasmid (general) 2 µg 100 µl 4D X-Unit
SF 96-EH-100 2e5 95.5% ±2 92.7% ±11 Plasmid (general) 0.4 µg 20 µl Shuttle
V T-028 1e6 34% ±14 83% ±10 Plasmid (general) 5 µg 100 µl I/II/2b
V T-028 1e6 64% ±7 86% ±7 Plasmid (general) 5 µg 100 µl I/II/2b

Citations (17)

Categories:
Transfection 
Authors:
Burda PC, Roelli MA, Schaffner M, Khan SM, Janse CJ, Heussler VT 
In:
PLoS Pathog (2015) 11(3): e1004760 
Categories:
Transfection 
Authors:
M Kuhlwilm, A Davierwala, S Pääbo 
In:
PLoS ONE (2013) 8 (12): e83218 
Categories:
Primary Cells and Media 
Authors:
Savary K, Caglayan D, Caja L, Tzavlaki K, Bin Nayeem S, Bergström T, Jiang Y, Uhrbom L, Forsberg-Nilsson K, Westermark B, Heldin CH, Ferletta M, Moustakas 
In:
Oncogene (2012) 32(47): 5409-5420 
Categories:
Transfection 
Authors:
Gao J, Zhao N, Knutson MD, Enns CA 
In:
J Biol Chem (2008) 283(31): 21462-8 
Categories:
Transfection 
Authors:
Phelan CA, Weaver JM, Steger DJ, Joshi S, Maslany JT, Collins JL, Zuercher WJ, Willson TM, Walker M, Jaye M, Lazar MA 
In:
Mol Endocrinol (2008) epub: online 
Categories:
Transfection 
Authors:
Zhang AS, Yang F, Meyer K, Hernandez C, Chapman-Arvedson T, Bjorkman PJ, Enns CA 
In:
J Biol Chem (2008) 283(25): 17494-502 
Categories:
Transfection 
Authors:
Nagle CA, Vergnes L, Dejong H, Wang S, Lewin TM, Reue K, Coleman RA 
In:
J Lipid Res (2008) 49(4): 823-31 
Categories:
Transfection 
Authors:
Yang C, Feng J, Song W, Wang J, Tsai B, Zhang Y, Scaringe WA, Hill KA, Margaritis P, High KA, Sommer SS 
In:
Proc Natl Acad Sci USA (2007) 104(39): 15394-9 
Categories:
Transfection 
Authors:
Kino T, Rice KC, Chrousos GP 
In:
Eur J Clin Invest (2007) 37(5): 425-33 
Categories:
Transfection 
Authors:
Zhang AS, Anderson SA, Meyers KR, Hernandez C, Eisenstein RS, Enns CA 
In:
J Biol Chem (2007) 282(17): 12547-56 
Categories:
Transfection 
Authors:
Gusarova GA, Wang IC, Major ML, Kalinichenko VV, Ackerson T, Petrovic V, Costa RH 
In:
J Clin Invest (2007) 117(1): 99-111 
Categories:
Transfection 
Authors:
Shetty S, Eckhardt ER, Post SR, van der Westhuyzen DR 
In:
Arterioscler Thromb Vasc Biol (2006) 26(9): 2125-31 
Categories:
Transfection 
Authors:
Alenghat T, Yu J, Lazar MA 
In:
EMBO J (2006) 25(17): 3966-74 
Categories:
Transfection 
Authors:
Citterio C, Jones HD, Pacheco-Rodriguez G, Islam A, Moss J, Vaughan M 
In:
Proc Natl Acad Sci USA (2006) 103(8): 2683-8 
Categories:
Transfection 
Authors:
Hirano KI, Ikegami C, Tsujii KI, Zhang Z, Matsuura F, Nakagawa-Toyama Y, Koseki M, Masuda D, Maruyama T, Shimomura I, Ueda Y and Yamashita S 
In:
Arterioscler Thromb Vasc Biol (2005) 25(11): 2422-2427 
Categories:
Transfection 
Authors:
Ding Q, Xia W, Liu JC, Yang JY, Lee DF, Xia J, Bartholomeusz G, Li Y, Pan Y, Li Z, Bargou RC, Qin J, Lai CC, Tsai FJ, Tsai CH and Hung MC 
In:
Mol Cell (2005) 19(2): 159-170 
Categories:
Transfection 
Authors:
Francova H, Trbusek M, Zapletalova P, Kuhrova V 
In:
J Inherit Metab Dis (2004) 27(4): 523-8 
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