Hep G2

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Human hepatocellular carcinoma cell line

Cell Type:
Liver Epith.
Tissue Origin:
liver
Species:
human
Research Area:
Cancer Research/Cell Biology
Gastroenterology
Toxicology
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SF EH-100 2e5 95.5% ±2 92.7% ±11 Plasmid (general) 0.4 µg 20 µl 96-well
SF EH-100 2e5 72.1% ±5 92% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SF EH-100 1e6 68.9% ±2 92% Plasmid (general) 2 µg 100 µl 4D X-Unit
V T-028 1e6 34% ±14 83% ±10 Plasmid (general) 5 µg 100 µl I/II/2b
V T-028 1e6 64% ±7 86% ±7 Plasmid (general) 5 µg 100 µl I/II/2b

Citations (24)

In vitro characterization of 3D culture-based differentiation of human liver stem cells
Categories:
Primary Cells and Media, 3D Cell Culture  
Authors:
Marta Tapparo , Gabriele Saccu , Chiara Pasquino , Valentina Fonsato , Claudio Medana , Valentina Schiavo , Enrica Mecarelli , Monica Maccagno , Lorenzo Silengo , Stefania Bruno , Giovanni Camussi , Maria Beatriz Herrera Sanchez  
In:
Frontiers in Pharmacology (2024) 12: 1352013 
Chemical manipulation of an activation/inhibition switch in the nuclear receptor PXR
Categories:
 
Authors:
Efren Garcia-Maldonado , Andrew D Huber , Sergio C Chai , Stanley Nithianantham , Yongtao Li , Jing Wu , Shyaron Poudel , Darcie J Miller , Jayaraman Seetharaman , Taosheng Chen  
In:
Nature (2024) 15: 4054 
Liver three-dimensional cellular models for high-throughput chemical testing
Categories:
3D Cell Culture  
Authors:
Shu Yang , Masato Ooka , Ryan Jared Margolis , Menghang Xia  
In:
Cell Rep (2023) 3: 100432 
Hepatitis Delta Virus Acts as an Immunogenic Adjuvant in Hepatitis B Virus-Infected Hepatocytes
Categories:
Primary Cells and Media, Transfection, Cell Lines and Primary Cancer Cells 
Authors:
Christine Y.L. Tham, Janine Kah, Anthony T. Tan, ..., Marc L€utgehetmann, Maura Dandri, Antonio Bertoletti 
In:
Cell Rep (2020) 1 (4): 100060 
Modulation of hepatitis B virus infection by epidermal growth factor secreted from liver sinusoidal endothelial cells
Categories:
Primary Cells and Media, Serum-free and Speciality Media, Cell Lines and Primary Cancer Cells 
Authors:
Shin-Wei C hen, Misao Himeno, Yuta Koui, Masaya Sugiyama, Hironori Nishitsuji, Masashi Mizokami, Kunitada Shimotohno, Atsushi Miyajima & Taketomo Kido 
In:
Scientific Reports (2020) 10: 14349 
TGF-ß inhibition via CRISPR promotes the long-term efficacy of CAR T cells against solid tumors
Categories:
Transfection 
Authors:
Tang N, Cheng C, Zhang X, Qiao M, Li N, Mu W, Wei XF, Han W, Wang H. 
In:
(2020) 27;5(4): e133977 
Generation of hepatobiliary organoids from human induced pluripotent stem cells
Categories:
 
Authors:
Fenfang Wu , Di Wu , Yong Ren , Yuhua Huang , Bo Feng , Nan Zhao , Taotao Zhang , Xiaoni Chen , Shangwu Chen , Anlong Xu  
In:
J Clin Transl Hepatol (2019) 70: 1145 
A Plasmodium phospholipase is involved in disruption of the liver stage parasitophorous vacuole membrane.
Categories:
Transfection 
Authors:
Burda PC, Roelli MA, Schaffner M, Khan SM, Janse CJ, Heussler VT 
In:
PLoS Pathog (2015) 11(3): e1004760 
Identification of Putative Target Genes of the Transcription Factor RUNX2
Categories:
Transfection 
Authors:
M Kuhlwilm, A Davierwala, S Pääbo 
In:
PLoS ONE (2013) 8 (12): e83218 
Snail depletes the tumorigenic potential of glioblastoma
Categories:
Primary Cells and Media 
Authors:
Savary K, Caglayan D, Caja L, Tzavlaki K, Bin Nayeem S, Bergström T, Jiang Y, Uhrbom L, Forsberg-Nilsson K, Westermark B, Heldin CH, Ferletta M, Moustakas 
In:
Oncogene (2012) 32(47): 5409-5420 
The hereditary hemochromatosis protein, HFE, inhibits iron uptake via downregulation of Zip14 in HEPG2 cells
Categories:
Transfection 
Authors:
Gao J, Zhao N, Knutson MD, Enns CA 
In:
J Biol Chem (2008) 283(31): 21462-8 
Selective Partial Agonism of Liver X Receptor alpha Is Related to Differential Corepressor Recruitment
Categories:
Transfection 
Authors:
Phelan CA, Weaver JM, Steger DJ, Joshi S, Maslany JT, Collins JL, Zuercher WJ, Willson TM, Walker M, Jaye M, Lazar MA 
In:
Mol Endocrinol (2008) epub: online 
Neogenin-mediated hemojuvelin shedding occurs after hemojuvelin traffics to the plamsa membrane
Categories:
Transfection 
Authors:
Zhang AS, Yang F, Meyer K, Hernandez C, Chapman-Arvedson T, Bjorkman PJ, Enns CA 
In:
J Biol Chem (2008) 283(25): 17494-502 
Identification of a novel sn-glycerol-3-phosphate acyltransferase isoform, GPAT4 as the enzyme deficient in Agpat6-/- mice
Categories:
Transfection 
Authors:
Nagle CA, Vergnes L, Dejong H, Wang S, Lewin TM, Reue K, Coleman RA 
In:
J Lipid Res (2008) 49(4): 823-31 
A mouse model for nonsense mutation bypass therapy shows a dramatic multiday response to geneticin
Categories:
Transfection 
Authors:
Yang C, Feng J, Song W, Wang J, Tsai B, Zhang Y, Scaringe WA, Hill KA, Margaritis P, High KA, Sommer SS 
In:
Proc Natl Acad Sci USA (2007) 104(39): 15394-9 
The PPARdelta agonist GW501516 suppresses interleukin-6-mediated hepatocyte acute phase reaction via STAT3 inhibition
Categories:
Transfection 
Authors:
Kino T, Rice KC, Chrousos GP 
In:
Eur J Clin Invest (2007) 37(5): 425-33 
Evidence that inhibition of hemojuvelin shedding in response to iron is mediated through neogenin
Categories:
Transfection 
Authors:
Zhang AS, Anderson SA, Meyers KR, Hernandez C, Eisenstein RS, Enns CA 
In:
J Biol Chem (2007) 282(17): 12547-56 
A cell-penetrating ARF peptide inhibitor of FoxM1 in mouse hepatocellular carcinoma treatment
Categories:
Transfection 
Authors:
Gusarova GA, Wang IC, Major ML, Kalinichenko VV, Ackerson T, Petrovic V, Costa RH 
In:
J Clin Invest (2007) 117(1): 99-111 
Phosphatidylinositol-3-Kinase Regulates Scavenger Receptor Class B Type I Subcellular Localization and Selective Lipid Uptake in Hepatocytes
Categories:
Transfection 
Authors:
Shetty S, Eckhardt ER, Post SR, van der Westhuyzen DR 
In:
Arterioscler Thromb Vasc Biol (2006) 26(9): 2125-31 
The N-CoR complex enables chromatin remodeler SNF2H to enhance repression by thyroid hormone receptor
Categories:
Transfection 
Authors:
Alenghat T, Yu J, Lazar MA 
In:
EMBO J (2006) 25(17): 3966-74 
Effect of protein kinase A on accumulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) in HepG2 cell nuclei
Categories:
Transfection 
Authors:
Citterio C, Jones HD, Pacheco-Rodriguez G, Islam A, Moss J, Vaughan M 
In:
Proc Natl Acad Sci USA (2006) 103(8): 2683-8 
Probucol enhances the expression of human hepatic scavenger receptor class B type I, possibly through a species-specific mechanism
Categories:
Transfection 
Authors:
Hirano KI, Ikegami C, Tsujii KI, Zhang Z, Matsuura F, Nakagawa-Toyama Y, Koseki M, Masuda D, Maruyama T, Shimomura I, Ueda Y and Yamashita S 
In:
Arterioscler Thromb Vasc Biol (2005) 25(11): 2422-2427 
Erk Associates with and Primes GSK-3beta for Its Inactivation Resulting in Upregulation of beta-Catenin
Categories:
Transfection 
Authors:
Ding Q, Xia W, Liu JC, Yang JY, Lee DF, Xia J, Bartholomeusz G, Li Y, Pan Y, Li Z, Bargou RC, Qin J, Lai CC, Tsai FJ, Tsai CH and Hung MC 
In:
Mol Cell (2005) 19(2): 159-170 
New promoter mutations in the low-density lipoprotein receptor gene which induce familial hypercholesterolaemia phenotype: molecular and functional analysis
Categories:
Transfection 
Authors:
Francova H, Trbusek M, Zapletalova P, Kuhrova V 
In:
J Inherit Metab Dis (2004) 27(4): 523-8 
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