GH3

pituitary tumor

Cell Type:
Pituitary Gland Epith.
Tissue Origin:
Species:
rat
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

UltraCULTURE™ Medium is a complete all purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines.
The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution (Cat. No. 17-605) per 500 ml.

The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium does not contain L-glutamine.

UltraCULTURE™ Medium may be supplemented with Cryoprotective Medium (Cat. No. 12-132) to cryopreserve cells in a serum-free environment.

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE DS-131 4e5 73-81% 58-78% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE DS-131 2e6 73-82% 55-77% Plasmid (general) 2 µg 100 µl 4D X-Unit
L T-005 1e6 75-79% 73-95% Plasmid (general) 2 µg 100 µl I/II/2b

Citations

Categories:
Transfection 
Authors:
Kino T, Tiulpakov A, Ichijo T, Chheng L, Kozasa T and Chrousos GP 
In:
J Cell Biol (2005) 169(6): 885-896