Melanocyte, (NHEM-Ad), human adult

Pigment producing epidermal cells, derived from adult skin tissue

Cell Type:
Skin/Hair Epith.
Tissue Origin:
dermal
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Suspension

Recommended Media

MGM-4 has been optimized for the growth and proliferation of normal human primary melanocytes in culture. This medium system has been shown to deliver superior results as compared to other existing commercial media systems. Melanocytes in culture maintain >90% functionality based on the conversion of L-dopa to dopa-melanin. The melanocyte media system also allows for normal morphology and proliferative capacity after recovery from cryopreservation and throughout serial passaging.

Adult melanocytes also require the addition of Et-3 (CC-4510), sold separately

Transfection Information

In case no data are shown for the selected cell type, please take a look at our optimization strategy or contact our Scientific Support Teams to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Citations (22)

Categories:
Serum-free and Speciality Media 
Authors:
Susanna Lo¨ nnqvist, Johan P. E. JunkerID, Maria Sedell1, Erika Nyman,Gunnar KratzID 
In:
PLoS ONE (2019) 8: e0221878 
Categories:
Primary Cells and Media 
Authors:
Bultema JJ, Boyle JA, Malenke PB, Martin FE, Dell\\\'Angelica EC, Cheney RE, Di Pietro SM 
In:
J Biol Chem (2014) 16: 16 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Tringali C, Silvestri I, Testa F, Baldassari P, Anastasia L, Mortarini R, Anichini A, López-Requena A, Tettamanti G, Venerando B 
In:
BMC Cancer (2014) 14: 560 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Fernández-Barral A, Orgaz JL, Baquero P, Ali Z, Moreno A, Tiana M, Gómez V, Riveiro-Falkenbach E, Cañadas C, Zazo S, Bertolotto C, Davidson I, Rodríguez-Peralto JL, Palmero I, Rojo F, Jensen LD, del Peso L, Jiménez B. 
In:
Neoplasia (2014) 16(6): 529-42 
Categories:
Primary Cells and Media 
Authors:
Fontana JM, Mygatt JG, Conant KL, Parsons CH, Kaleeba JA 
In:
J Skin Cancer (2014) 2014: 246076 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Kim TK1, Kleszczynski K, Janjetovic Z, Sweatman T, Lin Z, Li W, Reiter RJ, Fischer TW, Slominski AT. 
In:
FASEB J (2013) 27(7): 2742-55 
Categories:
Transfection 
Authors:
Cheung M, Sharma A, Madhunapantula SV, Robertson GP 
In:
Cancer Res (2008) 68(9): 3429-39 
Categories:
Transfection 
Authors:
Palmer MB, Majumder P, Green MR, Wade PA, Boss JM 
In:
Cancer Res (2007) 67(13): 6113-20 
Categories:
Transfection 
Authors:
Jordens I, Westbroek W, Marsman M, Rocha N, Mommaas M, Huizing M, Lambert J, Naeyaert JM, Neefjes J 
In:
Pigment Cell Res (2006) 19(5): 412-23 
Categories:
Transfection 
Authors:
Dumaz N, Hayward R, Martin J, Ogilvie L, Hedley D, Curtin JA, Bastian BC, Springer C, Marais R 
In:
Cancer Res (2006) 66(19): 9483-91 
Categories:
Transfection 
Authors:
Rouzaud F, Costin GE, Yamaguchi Y, Valencia JC, Berens WF, Chen KG, Hoashi T, Bohm M, Abdel-Malek ZA, Hearing VJ 
In:
FASEB J (2006) 20(11): 1927-9 
Categories:
Transfection 
Authors:
Gray-Schopfer VC, Cheong SC, Chong H, Chow J, Moss T, Abdel-Malek ZA, Marais R, Wynford-Thomas D, Bennett DC 
In:
Br J Cancer (2006) 95(4): 496-505 
Categories:
Transfection 
Authors:
Berens W, Van Den Bossche K, Yoon TJ, Westbroek W, Valencia JC, Out CJ, Marie Naeyaert J, Hearing VJ and Lambert J 
In:
Pigment Cell Res (2005) 18(5): 370-381 
Categories:
Transfection 
Authors:
Helip-Wooley A, Westbroek W, Dorward H, Mommaas M, Boissy RE, Gahl WA, Huizing M 
In:
BMC Cell Biol (2005) 6: 33 
Categories:
Transfection 
Authors:
Alkhateeb A, Fain PR and Spritz RA 
In:
J Invest Dermatol (2005) 125(2): 388-391 
Categories:
Transfection 
Authors:
Wellbrock C and Marais R 
In:
J Cell Biol (2005) 170(5): 703-708 
Categories:
Transfection 
Authors:
Bhatt KV, Spofford LS, Aram G, McMullen M, Pumiglia K and Aplin AE 
In:
Oncogene (2005) 24(21): 3459-3471 
Categories:
Transfection 
Authors:
Westbroek W, Lambert J, Schepper SD, Kleta R, Bossche KV, Seabra MC, Huizing M, Mommaas M and Naeyaert JM 
In:
Pigment Cell Res (2004) 17(5): 498-505 
Categories:
Transfection 
Authors:
Stahl JM, Sharma A, Cheung M, Zimmerman M, Cheng JQ, Bosenberg MW, Kester M, Sandirasegarane L and Robertson GP 
In:
Cancer Res (2004) 64(19): 7002-7010 
Categories:
Transfection 
Authors:
Yamaguchi Y, Itami S, Watabe H, Yasumoto K, Abdel-Malek ZA, Kubo T, Rouzaud F, Tanemura A, Yoshikawa K and Hearing VJ 
In:
J Cell Biol (2004) 165(2): 275-285 
Categories:
Transfection 
Authors:
Westbroek W, Lambert J, Bahadoran P, Busca R, Herteleer MC, Smit N, Mommaas M, Ballotti R and Naeyaert JM 
In:
J Invest Dermatol (2003) 120(3): 465-475 
Categories:
Transfection 
Authors:
Hamm A, Krott N, Breibach I, Blindt R and Bosserhoff AK 
In:
Tissue Eng (2002) 8(2): 235-245 
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