SMC, aortic (AoSMC), mouse

primary mouse SMC

Cell Type:
Smooth muscle cell
Tissue Origin:
aortic
Species:
mouse
Research Area:
Cancer Research/Cell Biology
Cardiovascular
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
Smooth muscle, basic X-013 1e6 30-35% 99% Plasmid (general) 2 µg 100 µl I/II/2b

Citations (12)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Sergei Paylian 
In:
BAOJ Cancer Research & Therapy (2015) 1(2): 1 
Categories:
Primary Cells and Media 
Authors:
Yang L, Sakurai T, Kamiyoshi A, Ichikawa-Shindo Y, Kawate H, Yoshizawa T, Koyama T, Iesato Y, Uetake R, Yamauchi A, Tanaka M, Toriyama Y, Igarashi K, Shindo T. 
In:
Other (2013) 59(2013): 55-66 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Davis R, Pillai S, Lawrence N, Sebti S, Chellappan SP. 
In:
Cell Cycle. (2012) 11 (1): 109-118 
Categories:
Transfection 
Authors:
Hui S, Choi J, Zaidi S, Momen A, Steinbach SK, Sadi AM, Ban K, Husain M. 
In:
Circ Res (2011) 108 (9): 1053-62 
Categories:
Transfection 
Authors:
Villeneuve LM, Reddy MA, Lanting LL, Wang M, Meng L, Natarajan R 
In:
Proc Natl Acad Sci USA (2008) 105(26): 9047-9052 
Categories:
Transfection 
Authors:
Yu N, Erb L, Shivaji R, Weisman GA, Seye CI 
In:
Circ Res (2008) 102(5): 581-8 
Categories:
Transfection 
Authors:
Tang RH, Zheng XL, Callis TE, Stansfield WE, He J, Baldwin AS, Wang DZ, Selzman CH 
In:
Proc Natl Acad Sci USA (2008) 105(9): 3362-7 
Categories:
Transfection 
Authors:
Cheng J, Zhang J, Merched A, Zhang L, Zhang P, Truong L, Boriek AM, Du J 
In:
J Biol Chem (2007) 282(47): 34268-75 
Categories:
Transfection 
Authors:
Yasuda O, Fukuo K, Sun X, Nishitani M, Yotsui T, Higuchi M, Suzuki T, Rakugi H, Smithies O, Maeda N, Ogihara T 
In:
J Biol Chem (2006) 281(33): 23899-23907 
Categories:
Transfection 
Authors:
Hou R, Liu L, Anees S, Hiroyasu S, Sibinga NE 
In:
J Cell Biol (2006) 173(3): 417-29 
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