COS-7

African green monkey kidney cell line; derived from CV-1 by transformation with origin defective SV-40 mutant

Cell Type:
Fibroblast
Tissue Origin:
kidney
Species:
monkey
Research Area:
Cancer Research/Cell Biology
Cell Characteristics:
Adherent

Recommended Media

UltraCULTURE™ Medium is a complete all purpose serum-free medium which supports the growth of a wide variety of both non-adherent and adherent cell lines.
The product is ready-to-use with the simple addition of 5.0 ml of L-glutamine solution (Cat. No. 17-605) per 500 ml.

The medium consists of a DMEM:F-12 base which is supplemented with recombinant human insulin, bovine transferrin and a purified mixture of bovine serum proteins including albumin. The total protein concentration of UltraCULTURE™ Medium is approximately 3 mg/ml. UltraCULTURE™ Medium does not contain L-glutamine.

UltraCULTURE™ Medium may be supplemented with Cryoprotective Medium (Cat. No. 12-132) to cryopreserve cells in a serum-free environment.

Storage = 2ºC to 8ºC

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE CM-130 2e5 90% 83% ±4 Plasmid (general) 0.4 µg 20 µl 4D X-Unit
SE CM-130 1e6 89% ±1 86% ±1 Plasmid (general) 2 µg 100 µl 4D X-Unit
R W-001 1e6 99.3% ± 93.9% ±4 Plasmid (general) 5 µg 100 µl I/II/2b

Citations (22)

Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Knapek KJ 
In:
Other (2008) 0:0: Thesis 
Categories:
Transfection 
Authors:
Nagle CA, Vergnes L, Dejong H, Wang S, Lewin TM, Reue K, Coleman RA 
In:
J Lipid Res (2008) 49(4): 823-31 
Categories:
Transfection 
Authors:
Wang C, Kovanen V, Raudasoja P, Eskelinen S, Pospiech H, Myllylä R 
In:
J Cell Mol Med (2008) epub: online 
Categories:
Transfection 
Authors:
Friese MA, Craner MJ, Etzensperger R, Vergo S, Wemmie JA, Welsh MJ, Vincent A, Fugger L 
In:
Nat Med (2007) 13(12): 1483-9 
Categories:
Transfection 
Authors:
Rider L, Shatrova A, Feener EP, Webb L, Diakonova M 
In:
J Biol Chem (2007) 282(42): 30985-96 
Categories:
Transfection 
Authors:
Liu P, Sudhaharan T, Koh RM, Hwang LC, Ahmed S, Maruyama IN, Wohland T 
In:
Biophys J (2007) 93(2): 684-98 
Categories:
Transfection 
Authors:
Li F, Papworth M, Minczuk M, Rohde C, Zhang Y, Ragozin S, Jeltsch A 
In:
Nucleic Acids Res (2007) 35(1): 100-112 
Categories:
Transfection 
Authors:
Schiller M, Bohm M, Dennler S, Ehrchen JM, Mauviel A 
In:
Oncogene (2006) 25(32): 4449-57 
Categories:
Transfection 
Authors:
Yoshimi R, Yamaji S, Suzuki A, Mishima W, Okamura M, Obana T, Matsuda C, Miwa Y, Ohno S, Ishigatsubo Y 
In:
J Immunol (2006) 176(6): 3611-24 
Categories:
Transfection 
Authors:
Khanday FA, Yamamori T, Mattagajasingh I, Zhang Z, Bugayenko A, Naqvi A, Santhanam L, Nabi N, Kasuno K, Day BW and Irani K 
In:
Mol Biol Cell (2006) 17(1): 122-129 
Categories:
Transfection 
Authors:
Cheng L, Lemmon S and Lemmon V 
In:
J Neurochem (2005) 94(4): 1102-1110 
Categories:
Transfection 
Authors:
Mango R, Biocca S, Del Vecchio F, Clementi F, Sangiuolo F, Amati F, Filareto A, Grelli S, Spitalieri P, Filesi I, Favalli C, Lauro R, Mehta JL, Romeo F and Novelli G 
In:
Circ Res (2005) 97(2): 152-158 
Categories:
Transfection 
Authors:
Romero X, Zapater N, Calvo M, Kalko SG, de la Fuente MA, Tovar V, Ockeloen C, Pizcueta P, Engel P 
In:
J Immunol (2005) 174(11): 7033-7042 
Categories:
Transfection 
Authors:
Salomonsen J, Sorensen MR, Marston DA, Rogers SL, Collen T, van Hateren A, Smith AL, Beal RK, Skjodt K, Kaufman J 
In:
Proc Natl Acad Sci USA (2005) 102(24): 8668-8673 
Categories:
Transfection 
Authors:
De Domenico I, Ward DM, Nemeth E, Vaughn MB, Musci G, Ganz T and Kaplan J 
In:
Proc Natl Acad Sci USA (2005) 102(25): 8955-8960 
Categories:
Transfection 
Authors:
Bao J, Jana SS and Adelstein RS 
In:
J Biol Chem (2005) 280(20): 19594-19599 
Categories:
Transfection 
Authors:
Martin M, Del Valle JM, Saborit I and Engel P 
In:
J Immunol (2005) 174(10): 5977-5986 
Categories:
Transfection 
Authors:
Olesen SH, Christensen LL, Sorensen FB, Cabezon T, Laurberg S, Orntoft TF and Birkenkamp-Demtroder K 
In:
Mol Cell Proteomics (2005) 4(4): 534-544 
Categories:
Transfection 
Authors:
Pratt SJ, Epple H, Ward M, Feng Y, Braga VM and Longmore GD 
In:
J Cell Biol (2005) 168(5): 813-824 
Categories:
Transfection 
Authors:
Lindvall JM, Blomberg KE, Valiaho J, Vargas L, Heinonen JE, Berglof A, Mohamed AJ, Nore BF, Vihinen M and Smith CI 
In:
Immunol Rev (2005) 203: 200-215 
Categories:
Transfection 
Authors:
Taguchi Y, Kondo T, Watanabe M, Miyaji M, Umehara H, Kotutumi Y, Okazaki T 
In:
Blood (2004) 104(10): 3285-3293 
Categories:
Transfection 
Authors:
Savaskan NE, Brauer AU and Nitsch R 
In:
Eur J Neurosci (2004) 19(1): 212-220 
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