cells

melonoma

Cell Type:

Skin/Hair Epith.

Tissue Origin:

dermal

Species:

human

Research Area:

Cancer Research/Cell Biology

Dermatology/Tissue Engineering

Cell Characteristics:

Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline

Filter:

The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.

Protocol	Kit	Program	Cells	Efficiency	Viable Cells	Substrate		Format	Platform
	R	U-020	1e6	25%	75%	Plasmid (general)	4.5 µg	100 µl	I/II/2b
	R	T-020	1e6	30%	60%	Plasmid (general)	4.5 µg	100 µl	I/II/2b

Citations

Akt3 and mutant V600E B-Raf cooperate to promote early melanoma development

Categories:

Transfection

Authors:

Cheung M, Sharma A, Madhunapantula SV, Robertson GP

In:

Cancer Res (2008) 68(9): 3429-39

Differential cellular responses to exogenous DNA in mammalian cells and its effect on oligonucleotide-directed gene modification

Categories:

Transfection

Authors:

Igoucheva O, Alexeev V and Yoon K

In:

Gene Ther (2006) 13(3): 266-75

Deregulated Akt3 activity promotes development of malignant melanoma

Categories:

Transfection

Authors:

Stahl JM, Sharma A, Cheung M, Zimmerman M, Cheng JQ, Bosenberg MW, Kester M, Sandirasegarane L and Robertson GP

In:

Cancer Res (2004) 64(19): 7002-7010

WM35

Transfection Information

Citations