REH
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human lymphoblastic leukemia cell line (non-T, non-B), derived from ALL patient
Cell Type:
PBMC
Tissue Origin:
blood
Species:
human
Research Area:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Cell Characteristics:
Suspension
Transfection Information
Lonza Optimized Protocol
Optimization Guideline
Filter:
Any Substrate
Any Platform
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols
or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our
optimization strategy
to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol
Kit
Program
Cells
Efficiency
Viable Cells
Substrate
Format
Platform
R
G-009
5e6
30%
70%
Plasmid (general)
2 µg
100 µl
I/II/2b
R
O-009
5e6
47%
50%
Plasmid (general)
2 µg
100 µl
I/II/2b
R
O-013
2e6
67%
90%
Plasmid (general)
2 µg
100 µl
I/II/2b
R
I-009
4e6
68%
50%
Plasmid (general)
2 µg
100 µl
I/II/2b
R
G-009
4e6
55%
69%
Plasmid (general)
2 µg
100 µl
I/II/2b
SF
CA-137
2e5
71-75%
63-67%
Plasmid (general)
1 µg
20 µl
4D X-Unit
SF
CA-137
1e6
82-86%
57-67%
Plasmid (general)
5 µg
100 µl
4D X-Unit
Citations
Expression of SMARCB1 modulates steroid sensitivity in human lymphoblastoid cells: identification of a promoter snp that alters PARP1 binding and SMARCB1 expression
Categories:
Transfection
Authors:
Pottier N, Cheok MH, Yang W, Assem M, Tracey L, Obenauer JC, Panetta JC, Relling MV, Evans WE
In:
Hum Mol Genet (2007) 16(19): 2261-71
Analysis of folylpoly-gamma-glutamate synthetase gene expression in human B-precursor ALL and T-lineage ALL cells
Categories:
Transfection
Authors:
Leclerc GJ, Leclerc GM, Kinser TT, Barredo JC
In:
BMC Cancer (2006) 6: 132
Degradation of cyclin D3 independent of Thr-283 phosphorylation
Categories:
Transfection
Authors:
Lahne HU, Kloster MM, Lefdal S, Blomhoff HK, Naderi S
In:
Oncogene (2006) 25(17): 2468-76