MRC-5

Fibroblast cell line derived from normal lung tissue of a 14-week-old male fetus by J.P. Jacobs (1966).

Cell Type:
Fibroblast
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

PC-1™ is a low-protein, serum-free medium intended for the culture of primary cells and anchorage-dependent cell lines. PC-1 is formulated in a specially modified DMEM/F12 base and contains a complete HEPES buffering system with known amounts of insulin, transferrin, fatty acids, and proprietary proteins assembled under strict quality control procedures. PC-1™ is intended for a variety of research and industrial applications and is formulated using defined components for optimal cell growth, while maintaining the lowest possible protein content.
PC-1™ does not contain L-glutamine.

PC-1™ Liquid Base Medium is to be stored at 2°C-8°C.
The Supplement should be stored at -20°C.
When these two components are combined, the resulting PC-1™ Complete Medium is stable for 45 days at 2°C-8°C.

Once thawed, the appropriate volume of one vial of PC-1™ Supplement must be combined with the companion volume of PC-1™ Liquid Base Medium. Partial reconstitution or repeated freezing and thawing of the PC-1™ Supplement is not advised.

UltraMEM Reduced Serum Medium is a chemically-defined medium designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations. When supplemented with 2-4% serum, UltraMEM Medium growth performance is comparable and, in some instances, superior to that of standard media supplemented with 10% fetal bovine serum.

Growth performance in UltraMEM can be further increased by addition of insulin, transferrin, selenium, and ethanolamine [ITS or ITES] to the basal medium.

Storage = 2ºC to 8ºC 

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE DC-100 5e5 80-90% 70-80% Plasmid (general) 2 µg 100 µl 4D X-Unit
SE DC-100 1e5 80-90% 60-70% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
R V-020 8e5 80-90% 80% Plasmid (general) µg 100 µl I/II/2b
R U-023 8e5 80% 80% Plasmid (general) µg 100 µl I/II/2b
Fibroblast, basic U-023 1e6 70% 50% Plasmid (general) 2 µg 100 µl I/II/2b
V X-005 1e6 84% 80% Plasmid (general) 1 µg 100 µl I/II/2b
V D-023 1e6 85% 80% Plasmid (general) 1 µg 100 µl I/II/2b
V T-030 1e6 92% 70% Plasmid (general) 1 µg 100 µl I/II/2b

Citations (7)

Categories:
Cell Lines and Primary Cancer Cells 
Authors:
Markus Hoffmann  1 , Hannah Kleine-Weber  2 , Simon Schroeder  3 , Nadine Krüger  4 , Tanja Herrler  5 , Sandra Erichsen  6 , Tobias S Schiergens  7 , Georg Herrler  8 , Nai-Huei Wu  8 , Andreas Nitsche  9 , Marcel A Müller  10 , Christian Drosten  3 , Stefan Pöhlmann  11              
In:
Cell (2020) 1: 1 
Categories:
Transfection 
Authors:
Li Q, Wilkie AR, Weller M, Liu X, Cohen JI. 
In:
PLoS Pathog (2015) 11(7): 1-26 
Categories:
Transfection 
Authors:
Machesky NJ, Zhang G, Raghavan B, Zimmerman P, Kelly SL, Merrill AH Jr, Waldman WJ, Van Brocklyn JR, Trgovcich J 
In:
J Biol Chem (2008) epub: online 
Categories:
Transfection 
Authors:
McNally BA, Trgovcich J, Maul GG, Liu Y, Zheng P 
In:
PLoS ONE (2008) 3(5): e2277 
Categories:
Transfection 
Authors:
Li Q, Ali MA, Cohen JI 
In:
Cell (2006) 127(2): 305-16 
Categories:
Transfection 
Authors:
Srsen V, Gnadt N, Dammermann A, Merdes A 
In:
J Cell Biol (2006) 174(5): 625-30 
Categories:
Transfection 
Authors:
Li C, Zienkiewicz J and Hawiger J 
In:
J Biol Chem (2005) 280(28): 26152-26159 
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