Fibroblast cell line derived from normal lung tissue of a 14-week-old male fetus by J.P. Jacobs (1966).
PC-1™ is a low-protein, serum-free medium intended for the culture of primary cells and anchorage-dependent cell lines. PC-1 is formulated in a specially modified DMEM/F12 base and contains a complete HEPES buffering system with known amounts of insulin, transferrin, fatty acids, and proprietary proteins assembled under strict quality control procedures. PC-1™ is intended for a variety of research and industrial applications and is formulated using defined components for optimal cell growth, while maintaining the lowest possible protein content.PC-1™ does not contain L-glutamine.PC-1™ Liquid Base Medium is to be stored at 2°C-8°C.The Supplement should be stored at -20°C.When these two components are combined, the resulting PC-1™ Complete Medium is stable for 45 days at 2°C-8°C.Once thawed, the appropriate volume of one vial of PC-1™ Supplement must be combined with the companion volume of PC-1™ Liquid Base Medium. Partial reconstitution or repeated freezing and thawing of the PC-1™ Supplement is not advised.
UltraMEM Reduced Serum Medium is a chemically-defined medium designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations. When supplemented with 2-4% serum, UltraMEM Medium growth performance is comparable and, in some instances, superior to that of standard media supplemented with 10% fetal bovine serum.Growth performance in UltraMEM can be further increased by addition of insulin, transferrin, selenium, and ethanolamine [ITS or ITES] to the basal medium.Storage = 2ºC to 8ºC
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