MRC-5

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Fibroblast cell line derived from normal lung tissue of a 14-week-old male fetus by J.P. Jacobs (1966).

Cell Type:
Fibroblast
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
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The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE DC-100 5e5 80-90% 70-80% Plasmid (general) 2 µg 100 µl 4D X-Unit
SE DC-100 1e5 80-90% 60-70% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
R V-020 8e5 80-90% 80% Plasmid (general) µg 100 µl I/II/2b
R U-023 8e5 80% 80% Plasmid (general) µg 100 µl I/II/2b
Fibroblast, basic U-023 1e6 70% 50% Plasmid (general) 2 µg 100 µl I/II/2b
V X-005 1e6 84% 80% Plasmid (general) 1 µg 100 µl I/II/2b
V D-023 1e6 85% 80% Plasmid (general) 1 µg 100 µl I/II/2b
V T-030 1e6 92% 70% Plasmid (general) 1 µg 100 µl I/II/2b

Citations (7)

SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor 
Categories:
Cell Lines and Primary Cancer Cells 
Authors:
Markus Hoffmann  1 , Hannah Kleine-Weber  2 , Simon Schroeder  3 , Nadine Krüger  4 , Tanja Herrler  5 , Sandra Erichsen  6 , Tobias S Schiergens  7 , Georg Herrler  8 , Nai-Huei Wu  8 , Andreas Nitsche  9 , Marcel A Müller  10 , Christian Drosten  3 , Stefan Pöhlmann  11              
In:
Cell (2020) 1: 1 
THY-1 Cell Surface Antigen (CD90) Has an Important Role in the Initial Stage of Human Cytomegalovirus Infection.
Categories:
Transfection 
Authors:
Li Q, Wilkie AR, Weller M, Liu X, Cohen JI. 
In:
PLoS Pathog (2015) 11(7): 1-26 
Human cytomegalovirus regulates bioactive sphingolipids
Categories:
Transfection 
Authors:
Machesky NJ, Zhang G, Raghavan B, Zimmerman P, Kelly SL, Merrill AH Jr, Waldman WJ, Van Brocklyn JR, Trgovcich J 
In:
J Biol Chem (2008) epub: online 
A role for cytoplasmic PML in cellular resistance to viral infection
Categories:
Transfection 
Authors:
McNally BA, Trgovcich J, Maul GG, Liu Y, Zheng P 
In:
PLoS ONE (2008) 3(5): e2277 
Insulin Degrading Enzyme Is a Cellular Receptor Mediating Varicella-Zoster Virus Infection and Cell-to-Cell Spread
Categories:
Transfection 
Authors:
Li Q, Ali MA, Cohen JI 
In:
Cell (2006) 127(2): 305-16 
Inhibition of centrosome protein assembly leads to p53-dependent exit from the cell cycle
Categories:
Transfection 
Authors:
Srsen V, Gnadt N, Dammermann A, Merdes A 
In:
J Cell Biol (2006) 174(5): 625-30 
Interactive sites in the MyD88 Toll/interleukin (IL) 1 receptor domain responsible for coupling to the IL1beta signaling pathway
Categories:
Transfection 
Authors:
Li C, Zienkiewicz J and Hawiger J 
In:
J Biol Chem (2005) 280(28): 26152-26159 
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