MRC-5

Fibroblast cell line derived from normal lung tissue of a 14-week-old male fetus by J.P. Jacobs (1966).

Cell Type:
Fibroblast
Tissue Origin:
lung
Species:
human
Research Area:
Cancer Research/Cell Biology
Dermatology/Tissue Engineering
Cell Characteristics:
Adherent

Recommended Media

UltraMEM Reduced Serum Medium is a chemically-defined medium designed to support growth and maintenance of several anchorage-dependent cell types under reduced serum concentrations. When supplemented with 2-4% serum, UltraMEM Medium growth performance is comparable and, in some instances, superior to that of standard media supplemented with 10% fetal bovine serum.

Growth performance in UltraMEM can be further increased by addition of insulin, transferrin, selenium, and ethanolamine [ITS or ITES] to the basal medium.

Storage = 2ºC to 8ºC 

Transfection Information

Lonza Optimized Protocol
Optimization Guideline
Filter:
The table below shows data for the cell type and Nucleofector™ Platform selected. Those data are either based on Lonza Optimized Protocols or on results shared from customers who performed an optimization based on our guidelines. In case no data are shown for the selected Nucleofector™ Platform, please take a look at our optimization strategy to get further guidance on how to easily determine optimal Nucleofection conditions yourself.
Protocol Kit Program Cells Efficiency Viable Cells Substrate Format Platform
SE DC-100 5e5 80-90% 70-80% Plasmid (general) 2 µg 100 µl 4D X-Unit
SE DC-100 1e5 80-90% 60-70% Plasmid (general) 0.4 µg 20 µl 4D X-Unit
R V-020 8e5 80-90% 80% Plasmid (general) µg 100 µl I/II/2b
R U-023 8e5 80% 80% Plasmid (general) µg 100 µl I/II/2b
Fibroblast, basic U-023 1e6 70% 50% Plasmid (general) 2 µg 100 µl I/II/2b
V X-005 1e6 84% 80% Plasmid (general) 1 µg 100 µl I/II/2b
V D-023 1e6 85% 80% Plasmid (general) 1 µg 100 µl I/II/2b
V T-030 1e6 92% 70% Plasmid (general) 1 µg 100 µl I/II/2b

Citations (7)

Categories:
Cell Lines and Primary Cancer Cells 
Authors:
Markus Hoffmann  1 , Hannah Kleine-Weber  2 , Simon Schroeder  3 , Nadine Krüger  4 , Tanja Herrler  5 , Sandra Erichsen  6 , Tobias S Schiergens  7 , Georg Herrler  8 , Nai-Huei Wu  8 , Andreas Nitsche  9 , Marcel A Müller  10 , Christian Drosten  3 , Stefan Pöhlmann  11              
In:
Cell (2020) 1: 1 
Categories:
Transfection 
Authors:
Li Q, Wilkie AR, Weller M, Liu X, Cohen JI. 
In:
PLoS Pathog (2015) 11(7): 1-26 
Categories:
Transfection 
Authors:
Machesky NJ, Zhang G, Raghavan B, Zimmerman P, Kelly SL, Merrill AH Jr, Waldman WJ, Van Brocklyn JR, Trgovcich J 
In:
J Biol Chem (2008) epub: online 
Categories:
Transfection 
Authors:
McNally BA, Trgovcich J, Maul GG, Liu Y, Zheng P 
In:
PLoS ONE (2008) 3(5): e2277 
Categories:
Transfection 
Authors:
Li Q, Ali MA, Cohen JI 
In:
Cell (2006) 127(2): 305-16 
Categories:
Transfection 
Authors:
Srsen V, Gnadt N, Dammermann A, Merdes A 
In:
J Cell Biol (2006) 174(5): 625-30 
Categories:
Transfection 
Authors:
Li C, Zienkiewicz J and Hawiger J 
In:
J Biol Chem (2005) 280(28): 26152-26159 
+ Show All